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Introduction to containment level 3 (biosafety level 3, BSL3) research laboratory, a hypothetical approach to identifying a potential bioterrorism agent that is classed as a hazard group three microorganism.

About This Simulation

In the Biosafety Simulation you will be introduced to the concept of laboratory containment, a very special type of laboratory required to work with hazardous pathogens. Containment in this context refers to the requirements set out by various bodies that are required to safely manipulate such pathogens.

In particular, in this simulation, you will be able to experience a containment level 3 (Biosafety level 3 or BSL3) research laboratory. You will become familiar with the basic protocols utilized there to ensure safety, for example, you will be presented with the specific basic safety rules for this type of laboratories (e.g. personal protective equipment (PPE) and primary containment). In addition, you will understand how every part of the laboratory is designed to avoid contamination (e.g. why the pressure in the laboratory needs to be controlled at all times (secondary containment), and how the microbiological safety cabinets are designed to offer protection).

In this simulation you will be presented with a hypothetical case where you will need to identify a potential bioterrorism agent that is classed as hazard group three microorganism.

“Note that this simulation has been developed broadly around UK regulations. Some protocols may differ depending on the specific regulations.”



Learning Objectives

  • Understand how a Biosafety containment level III laboratory is constructed (e.g. air flow and pressure control)
  • Understand the basic safety rules of a Biosafety containment level III laboratory (e.g. use of safety equipment)
  • Know about the basic rules for how to handle microorganisms in a Biosafety containment level III laboratory
  • Understand the concept of fumigation and how this is executed within a microbiological safety cabinet.

Techniques In This Simulation

  • BSL-3 sterile technique
  • Bacteria inoculation
  • Microscopy


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