Cell Culture Basics – Transfection Virtual Lab

Time to complete course: 39 min.

About the Cell Culture Basics - Transfection Virtual Lab Simulation

In the Cell Culture Basics – Transfection simulation, you will transfect a melanoma cell line using a lipid-based reagent in order to overexpress a gene that is involved in skin cancer. You will prepare the cells and reagents and use a DNA plasmid carrying the green fluorescent protein as reporter.

Will you be able to create fluorescent cells?

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Transfect a melanoma cell line using a lipid-based reagent in order to overexpress a gene that is involved in skin cancer.

Techniques In Lab

  • Cell culture
  • Cell transfection
  • Fluorescence microscopy

Learning Objectives

At the end of this simulation, you will be able to…

  • Explain the advantages of using lipidbased transfection methods
  • Design a transfection experiment:
    • Select the right transfection strategy
    • Correctly use controls
  • Describe and perform the key steps for transfecting mammalian cells using Lipofectamine 3000:
    • Prepare cells to achieve highest transfection efficiency (number of passage, percentage of confluence)
    • Prepare reagents (storing, handling, complexation time, incubation time)
  • Explain the molecular mechanism of lipidbased transfection
  • Troubleshoot poor transfection efficiency outcomes

Screenshots of Cell Culture Basics - Transfection Virtual Lab Simulation

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