Cell Culture Basics - Transfection

In this lab you will transfect a melanoma cell line using a lipid-based reagent in order to overexpress a gene that is involved in skin cancer.

Watch Video

Purchase Access

About This Simulation

In the Cell Culture basics – Transfection simulation you will transfect a melanoma cell line using a lipid-based reagent in order to overexpress a gene that is involved in skin cancer. You will prepare the cells and the reagents and you will use a DNA plasmid carrying the green fluorescent protein as reporter. Will you be able to get fluorescent cells?

Screenshots

Collaborators

Learning Objectives

Understanding the advantages of using lipid-based transfection methods
Understanding how to design a transfection experiment:
- Transfection strategies
- Use of controls
Learning the key steps for transfecting mammalian cells using Lipofectamine 3000:
- Preparation of cells to achieve highest transfection efficiency (number of passage, percentage of confluence)
- Preparation of reagents (storing, handling, complexation time, incubation time)
Understanding the molecular mechanism of lipid-based transfection
Learning how to solve poor transfection efficiency outcomes

Collaborator

List of all our learning simulations

View All Simulations