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Cell culture basics
In the Cell Culture Basics Simulation, you will use the aseptic technique to culture mammalian cells.

About This Simulation

In the Cell Culture Basics Simulation, you will use the aseptic technique to culture mammalian cells. You will perform a cell passage and freeze some cells for a backup stock. You will use microscopy to distinguish different cell morphologies and investigate how cells grow. Will you be able to maintain your cells alive?

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Learning Objectives

  • Understanding how to use the aseptic technique and other good laboratory practices in a cell culture lab
  • Understanding the minimum requirements to have and adequate cell environment that supports cell growth
  • Learning the key steps when working with mammalian cells in vitro
    • Thawing and plating
    • Cell passaging
    • Cell cryopreservation
  • Using different equipment, like a biosafety cabinet or an automated cell counter

Techniques

  • Cell culture
  • Cell passaging
  • Cell counting
  • Cell cryopreservation

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