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Cell Culture Basics

In the Cell Culture Basics Simulation, you will use the aseptic technique to culture mammalian cells.

About This Simulation

Cell culture refers to the removal of cells from an animal or plant and their subsequent growth in a favorable artificial environment. That is why cell culture is always an in vitro procedure. The cells may be removed from the tissue directly and disaggregated by enzymatic or mechanical means before cultivation, or they may be derived from a cell line or cell strain that has already been established.

In the Cell Culture Basics Simulation, you will use the aseptic technique to culture mammalian cells. You will perform a cell passage and freeze some cells for a backup stock. You will use microscopy to distinguish different cell morphologies and investigate how cells grow. Will you be able to maintain your cells alive?

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Learning Objectives

  • Understanding how to use the aseptic technique and other good laboratory practices in a cell culture lab
  • Understanding the minimum requirements to have and adequate cell environment that supports cell growth
  • Learning the key steps when working with mammalian cells in vitro
    • Thawing and plating
    • Cell passaging
    • Cell cryopreservation
  • Using different equipment, like a biosafety cabinet or an automated cell counter

Techniques In This Simulation

  • Cell culture
  • Cell passaging
  • Cell counting
  • Cell cryopreservation

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