Light Microscopy

Time to complete course: 20 min.

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About Light Microscopy Virtual Lab Simulation

This simulation, along with “Fluorescence Microscopy,” has been adapted from the original, larger “Microscopy” simulation.

Assemble the light microscope and discover how the key components help to magnify an image up to 1,000 times. In this simulation, you will learn how to use a light microscope to analyze an intestine tissue sample. You will discover why biological samples need to be processed before they can be imaged and what the applications and limitations of light microscopy in biology are. Your mission is to decide whether the Mallory staining can be used in the experiment your colleagues in the lab are proposing.

Assemble the microscope

First, you will assemble the light microscope and discover the function of the key components. Learn how, together, the parts of the microscope can magnify the image up to 1,000 times.

Analyze the sample

The sample is prepared for you already. You need to find out whether the staining used is appropriate for studying the microanatomy of the chicken intestine. Intestinal physiology specialist Dr. One will point out some interesting features to you as you explore the sample. Can you clearly distinguish all the cell types they are interested in? Finally, you and Dr. One will compare microscopy techniques. Is the light microscope the one microscope to rule them all?


  • Fluorescence Microscopy
  • Microscopy

Get Started Now

Enter the virtual microscope room to see inside a tissue sample. Learn how a light microscope can magnify an image and answer biological questions.

Techniques in Lab

  • Light microscopy

Learning Objectives

  • Understand the basic principles and practical aspects of light microscopy
    • Explain the function of different parts of the microscope
    • Compare the terms magnification, contrast, and resolution
    • Describe the application and limitations of light microscopy in biology
    • Understand the need for sample preparation

Simulation Features

Length – 20 minutes
Languages – English

Examples of Related Standards


Life Science related 1st year onwards


HS-PS1-3, HS-PS1-6


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10.2 Functional group chemistry
20.1 Types of organic reactions


Unit 2: Ionic Compounds Structure and Properties

Screenshots of Light Microscopy Virtual Lab Simulation


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