Mammalian Transient Protein Expression Virtual Lab

Time to complete course: 38 min.

About the Mammalian Transient Protein Expression Virtual Lab Simulation

In the Mammalian Transient Protein lab, you will use the ExpiCHO expression system to perform a transient protein production in ExpiCHO-S cells. You will grow a suspension culture, prepare both the reagents and the cells for transfection, and learn which protocol you should use, depending on the estimated protein yield. Will you be able to keep your cells healthy to maximize protein expression?

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Use the ExpiCHO expression system to perform a transient protein production in ExpiCHO-S cells.

Techniques In Lab

  • Cell culture
  • Cell transfection
  • Protein expression

Learning Objectives

At the end of this simulation, you will be able to…

  • Explain the benefits of using mammaliancell based expression systems to produce recombinant proteins compared to other systems based on other hosts, such as E. coli
  • Describe and perform the key steps for mammalian transient protein expression using the ExpiCHO protein expression kit:
    • Preparation of ExpiCHOS cells and transfection reagents
    • Transfection of cells
    • Addition of ExpiCHO Feeder and ExpiCHO Enhancer
  • Select the cell culture conditions to maximize protein expression yield

Screenshots of Mammalian Transient Protein Expression Virtual Lab Simulation

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