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Mammalian Transient Protein Expression

In the Mammalian Transient Protein simulation, you will use the ExpiCHO expression system to perform a transient protein production in ExpiCHO-S cells.

About This Simulation

In the Mammalian Transient Protein Simulation, you will use the ExpiCHO expression system to perform a transient protein production in ExpiCHO-S cells. You will grow a suspension culture, prepare both the reagents and the cells for transfection, and learn which protocol you should use, depending on the estimated protein yield. Will you be able to keep your cells healthy to maximize protein expression?

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Learning Objectives

  • Understanding that mammalian-cell based expresion generates recombinant proteins with more native folding and post┬átranslational modifications than other systems based on other hosts, such as E. coli
  • Learning the key steps for mammalian transient protein expression using the ExpiCHO protein expression kit:
    • Preparation of ExpiCHO-S cells and transfection reagents
    • Transfection of cells
    • Addition of ExpiCHO Feeder and ExpiCHO Enhancer
  • Understanding why it is important to maintain the suspension culture healthy in order to maximize protein expression yield

Techniques

  • Cell culture
  • Cell transfection
  • Protein expression

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