Pluripotent Stem Cell Culture
About the Pluripotent Stem Cell Culture Virtual Lab Simulation
In the Pluripotent Stem Cell Culture lab, you will culture and expand pluripotent stem cells (PSCs) using a feeder-free system. You will also evaluate the PSCs characteristic cell growth, solve differentiation-related issues and investigate the pluripotency of your PSCs using fluorescence microscopy. Will you be able to maintain the pluripotency of your culture?
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Techniques In Lab
- Cell culture
- Fluorescence microscopy
At the end of this simulation, you will be able to…
- Define the concept of pluripotent stem cell
- Describe the required combination of media and matrices that support cell health and pluripotency
- Describe and perform the critical steps for culturing PSCs:
- Thawing and plating
- Evaluating differentiated cells morphology and removing them from the culture
- Evaluating the pluripotency state of your PSCs using fluorescence microscopy
Screenshots of Pluripotent Stem Cell Culture Virtual Lab Simulation
How it works
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