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Pluripotent Stem Cell Culture

In the Pluripotent Stem Cell Culture simulation, you will culture and expand pluripotent stem cells (PSCs) using a feeder-free system.

About This Simulation

In the Pluripotent Stem Cell Culture Simulation, you will culture and expand pluripotent stem cells (PSCs) using a feeder-free system. You will also evaluate the PSCs characteristic cell growth, solve differentiation-related issues and investigate the pluripotency of your PSCs using fluorescence microscopy. Will you be able to maintain the pluripotency of your culture?

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Learning Objectives

  • Understanding the concept of pluripotent stem cell
  • Learning the required combination of media and matrices that support cell health and pluripotency
  • Learning the critical steps for culturing PSCs:
    • Thawing and plating
    • Evaluating differentiated cells morphology and learning how to remove them from the culture
    • Evaluating the pluripotency state of your PSCs using fluorescence microscopy

Techniques

  • Cell culture
  • Fluorescence microscopy

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