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Pluripotent Stem Cell Culture Virtual Lab

Time to complete course: 79 min.Need help? Visit our Help Center.

About the Pluripotent Stem Cell Culture Virtual Lab Simulation

In the Pluripotent Stem Cell Culture lab, you will culture and expand pluripotent stem cells (PSCs) using a feeder-free system. You will also evaluate the PSCs characteristic cell growth, solve differentiation-related issues and investigate the pluripotency of your PSCs using fluorescence microscopy. Will you be able to maintain the pluripotency of your culture?

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Culture and expand pluripotent stem cells (PSCs) using a feeder-free system.

Techniques In Lab

  • Cell culture
  • Fluorescence microscopy

Learning Objectives

At the end of this simulation, you will be able to…

  • Define the concept of pluripotent stem cell
  • Describe the required combination of media and matrices that support cell health and pluripotency
  • Describe and perform the critical steps for culturing PSCs:
    • Thawing and plating
    • Evaluating differentiated cells morphology and removing them from the culture
  • Evaluating the pluripotency state of your PSCs using fluorescence microscopy

Screenshots of Pluripotent Stem Cell Culture Virtual Lab Simulation

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How it works

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You will get unlimited access to all our simulations. We release new simulations nearly every month, and you can always see an overview of all our simulations in our simulation catalog.

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What are the minimum requirements for my computer?

You can find an overview of the minimum requirements here.

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