RNA Extraction

Learn how to extract RNA from pig fat tissue samples and how to purify messenger RNA using magnetic beads.

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  • Description
  • Features

How do you know which genes in your DNA are active in your cells? In this simulation, you will learn how to extract messenger RNA molecules, the direct product of your genes.

Extracting mRNA from pigs

You will first retrieve samples of fat tissue from pigs at a farm. Back in the lab, you will then break the cells to extract the total RNA. FInally, you will purify the messenger RNA from the rest of the RNA in your sample.

mRNA extraction and purification

First, the students will learn how to lyse cells, extract RNA using a chloroform solution and purify mRNA with magnetic beads.

Students are immediately provided with qualitative data (spectrophotometer, electrophoresis) to check the quality of their samples.

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Learn how to extract RNA from pig fat tissue samples and how to purify messenger RNA using magnetic beads.

Techniques in lab
Chloroform Extraction, Magnetic Beads Purification
Learning objectives

At the end of this simulation, you will be able to...

  • Understand how to extract the total RNA from a cell
  • Separate mRNA molecules specifically from the rest of the RNA
Simulation features

Length: 35
Accessibility mode: Not available
Languages: English (United States)

NGSS

No direct alignment

IB

No direct alignment

AP

No direct alignment