RNA Extraction: Sample and purify mRNA from pigs
About the RNA Extraction Virtual Lab Simulation
How do you know which genes in your DNA are active in your cells? In this simulation, you will learn how to extract messenger RNA molecules, the direct product of your genes.
Extracting mRNA from pigs
You will first retrieve samples of fat tissue from pigs at a farm. Back in the lab, you will then break the cells to extract the total RNA. FInally, you will purify the messenger RNA from the rest of the RNA in your sample.
mRNA extraction and purification
First, the students will learn how to lyse cells, extract RNA using a chloroform solution and purify mRNA with magnetic beads.
Students are immediately provided with qualitative data (spectrophotometer, electrophoresis) to check the quality of their samples.
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Techniques In Lab
- Chloroform Extraction
- Magnetic Beads Purification
At the end of this simulation, you will be able to…
- Understand how to extract the total RNA from a cell
- Separate mRNA molecules specifically from the rest of the RNA
Screenshots of RNA Extraction Virtual Lab Simulation
Prof. Susanna Cirera SalicioInstitute for Department of Basic Animal and Veterinary Sciences Faculty of Health and Medical Sciences, University of Copenhagen
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