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RNA Extraction: Sample and purify mRNA from pigs | Virtual Lab

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Higher Education
 
RNA Extraction: Sample and purify mRNA from pigs
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About This Simulation

Learn how to extract RNA from pig fat tissue samples and how to purify messenger RNA using magnetic beads.

Learning Objectives

  • Understand how to extract the total RNA from a cell
  • Separate mRNA molecules specifically from the rest of the RNA

About This Simulation

Level:
Higher Education
Length:
35
Min
Accessibility Mode:
Available
Languages:
English
French
Spanish
German

Lab Techniques

  • Chloroform Extraction
  • Magnetic Beads Purification
No lab techniques are listed for this simulation.

Related Standards

University:
NGSS:
  • No direct alignment
AP:
  • No direct alignment
LB:
  • No direct alignment
No lab techniques are listed for this simulation.

Learn More About This Simulation

How do you know which genes in your DNA are active in your cells? In this simulation, you will learn how to extract messenger RNA molecules, the direct product of your genes.

Extracting mRNA from pigs

You will first retrieve samples of fat tissue from pigs at a farm. Back in the lab, you will then break the cells to extract the total RNA. FInally, you will purify the messenger RNA from the rest of the RNA in your sample.

mRNA extraction and purification

First, the students will learn how to lyse cells, extract RNA using a chloroform solution and purify mRNA with magnetic beads.

Students are immediately provided with qualitative data (spectrophotometer, electrophoresis) to check the quality of their samples.

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Wenatchee Valley College

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