Thermo Fisher

Labster partners with Thermo Fisher to provide the following four simulations

The labs can be accessed for free via Thermo Fisher’s website by following the links below.

Cell Culture Basics Virtual Lab

About this simulation

Find out what it’s like to work in a cell culture laboratory. Learn how to use the aseptic technique to avoid contamination on your cells, and discover what it takes to keep them alive.

At the end of this simulation, you will be able to…

  • Apply the aseptic technique and other good laboratory practices in a cell culture lab
  • Describe the minimum requirements to have an adequate cell environment that supports cell growth
  • Describe and perform the key steps when working with mammalian cells in vitro: thawing and plating; cell passaging; cell cryopreservation
  • Correctly use a biosafety cabinet and an automated cell counter

Techniques

  • Cell culture
  • Cell passaging
  • Cell counting
  • Cell cryopreservation

Cell Culture Basics—Transfection Virtual Lab

About this simulation

Transfect a melanoma cell line using a lipid-based reagent in order to overexpress a gene that is involved in skin cancer.

At the end of this simulation, you will be able to…

  • Explain the advantages of using lipidbased transfection methods
  • Design a transfection experiment
  • Describe and perform the key steps for transfecting mammalian cells using Lipofectamine 3000
  • Explain the molecular mechanism of lipidbased transfection
  • Troubleshoot poor transfection efficiency outcomes

Techniques

  • Cell culture
  • Cell transfection
  • Fluorescence microscopy

Enter the Cell Culture Basics—Transfection Virtual Lab

Pluripotent Stem Cell Culture Virtual Lab

About this simulation

Culture and expand pluripotent stem cells (PSCs) using a feeder-free system.

At the end of this simulation, you will be able to…

  • Define the concept of pluripotent stem cell
  • Describe the required combination of media and matrices that support cell health and pluripotency
  • Describe and perform the critical steps for culturing PSCs
  • Evaluating the pluripotency state of your PSCs using fluorescence microscopy
Techniques
  • Cell culture
  • Fluorescence microscopy

Mammalian Transient Protein Expression Virtual Lab

About this simulation

Use the ExpiCHO expression system to perform a transient protein production in ExpiCHO-S cells.

At the end of this simulation, you will be able to…

  • Explain the benefits of using mammaliancell based expression systems to produce recombinant proteins compared to other systems based on other hosts, such as E. coli
  • Describe and perform the key steps for mammalian transient protein expression using the ExpiCHO protein expression kit:
  • Select the cell culture conditions to maximize protein expression yield

Techniques

  • Cell culture
  • Cell transfection
  • Protein expression

Enter the Mammalian Transient Protein Expression Virtual Lab

Cell culture
WxpiCHO-S cells in Shake flask
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