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Nuclear Magnetic Resonance (NMR): Analyze small protein samples Virtual Lab

In the Nuclear Magnetic Resonance simulation, you will learn how to use NMR to characterize binding events between proteins and ligands. You will perform NMR experiments and interpret the resulting spectra. Will you be able to help improve an existing antibiotic in order to reduce its side effects?

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Nuclear Magnetic Resonance (NMR): Analyze small protein samples

About this Simulation

Get your spin magnetized with NMR! In this simulation, you will learn about the nuclear magnetic resonance (NMR) technique from both a theoretical and practical perspective. In order to succeed, you will need to work with the instrument hands-on, preparing and running samples on it, and analyze the data.

Exploring protein-antibiotic interactions

Your mission will be to investigate how a common antibiotic may sometimes cause unwelcome side effects. After getting into the basics of how NMR works, you will explore how this antibiotic interacts with a protein in the kidneys, leading to kidney malfunction. Identifying the key interaction points is crucial in the work to improve the drug!

Running your own NMR samples

There are several distinct ways to use NMR. In your work in the virtual lab, we will discuss both isotope options, as well as 1-, 2- or 3-dimensional NMR. You will help decide which one is most suitable for the research questions we’re trying to answer. After preparing, loading and running your samples on the NMR instrument, you will need to interpret the produced spectra. Don’t worry, your lab assistant will be there to guide you!

Side effects that keep people sick

To complete your mission, you will have to understand the aspects of NMR well enough to identify the key interaction points between the kidney protein and the antibiotic so we can look into modifying these sites and avoid the interaction.

Will you be able to help reduce the side effects of this drug?

Learning Objectives

At the end of this simulation, you will be able to:

  • Understand how to perform NMR experiments

  • Understand how to analyze NMR spectra

Techniques in Lab

At the end of this simulation, you will be able to:

  • NMR

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