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5 Ways to Make ‘Inheritance with Punnett Square’ A More Approachable Topic

Akanksha Saxena
Teaching with Labster

Introduction: Inheritance with Punnett Square

Genetics and inheritance are amazing subjects that have enthralled human beings for almost 200 years now. Gregor Mendel, popularly known as the “father of genetics” worked for over 8 years on pea plants to lay out the basic plan of how genetics works and how it can be studied.

Various tools are commonly used to study inheritance patterns and genotype-phenotype relationships. The 2 most widely used tools are ‘pedigrees’ and ‘Punnett square’. You can check and use the Inheritance with Pedigree simulation from Labster to explain the first tool. The other one is Punnett square which we will discuss here.

A good understanding of the subject of genetics and inheritance can help in the prediction of different likelihoods of hereditary traits and diseases amongst parents and progeny. These prediction tools are widely used by both help plant and animal breeders and genetic consultants while developing better varieties and advising expecting couples about pregnancy outcomes respectively. After prediction, these tools are widely used to explain different patterns of inheritance in family lines.

For classroom lessons on Punnett square, we have compiled some resources here. We have highlighted all the major obstacles encountered by students when dealing with this important tool of genetic analysis. We also list some practical solutions that educators can use to solve those issues. By the end, we’ll convince you why a virtual lab simulation will prove useful not only for your students but also for you as an educator to deliver concepts more efficiently.

3 reasons why ‘Inheritance with Punnett Square’ can be tricky to teach or learn

There are 3 reasons why students are overwhelmed by the topic of Inheritance with Punnett Square. Acknowledging these issues is the first step toward making the topic more approachable. 

1. Confusion in basic genetics

The primary reason why most students face difficulty while dealing with the Punnett square analyses is a lack of understanding of basic genetics concepts. Many students don’t understand the primary laws of inheritance (Mendel’s laws of heredity). Most students are confused between the two laws: ‘The Law of Segregation and ‘The Law of Independent Assortment. Also, many students don’t understand the basic difference between “genes and alleles”, “character and character states/traits”, “autosomal and sex-linked traits”, “genotypes and phenotypes”, “homozygous and heterozygous genotypes”, “dominant and recessive alleles”, “affected, non-affected and carrier individuals”, etc. Without an a priori clear understanding of these typical concepts, it can be an extremely difficult task to comprehend and analyze inheritance patterns using pedigrees.

2. Failure to imbibe the novel tool’s intricacies

The Punnett square analysis is a methodology used for easy calculation of the mathematical probability of different trait inheritance. Though overall, it’s a very objective and easy way to calculate trait probabilities, it becomes challenging for students when they can’t follow its novel concept. Students are often observed struggling with the calculations of the odds of different offspring with different genotypes. Not knowing ‘how the dominant allele can express itself in both homozygous and heterozygous states while recessive allele is restricted to express itself only in homozygous state’ could be a very frustrating and disappointing situation to be in! Without knowing the basics of this, the Punnett square handling can be very challenging for students. 

3. Lack of practice and exciting demonstrations 

Solving Punnett squares requires students to be utterly attentive and have the guile to relate the genotype and phenotypic expressions. If students fail to pay undistracted attention at any point in time when educators solve these Punnett squares, it can become a very unpleasant topic to follow. This highlights the importance of engaging tools that can keep students amused and actively involved while educators are explaining how to predict the probable genetic constitutions of progenies based on the parents’ genotype and phenotype. Additionally, it becomes difficult for educators to handhold each student through every step of this analysis. This eventually reflects in the lack of practice and commonly encountered hesitation to approach Punnett square problems.

5 ways to make ‘Inheritance with Punnett Square’ a more approachable topic to understand

To address the complications encountered while teaching the ‘Inheritance with Punnett Square’, educators can engage the under-listed solutions in their classes. These can clarify many instrumental aspects of this genetic tool. Not only can they make teaching easier for educators like you but will also make lessons clearer and easier to assimilate for your students.

  1. Begin with the basics

It’s of prime importance that the basics of genetics and inheritance are clear to the students. As a first measure, educators should talk in depth about the following topics.

  • The idea of genetics and inheritance patterns of different traits and diseases. 

  • Mendelian genetics and inheritance 

  • The idea of the genetic locus 

  • Idea of ploidy (monoploid, diploid, etc)

  • 3 laws of heredity

  • Parent-progeny relationships, sibling relationships

  • The idea of genotypes and phenotype

  • Difference between genes and alleles

  • Difference between character and character states/traits

  • The idea of dominant and recessive alleles

  • The idea of autosomal and sex-linked traits

  • The idea of dominant and recessive traits

  • Idea of homozygosity and heterozygosity

  • The idea of pure-bred and crossbred

  • The idea of hybridization and monohybrid-vs-dihybrid crosses

Dominant and recessive allele

Figure: Difference between genotypes and phenotypes, dominant and recessive alleles, and their expressions. Image Source


Figure: Simple Punnett square explains the concept of ‘hybridization’. Image Source

2. Help them understand and differentiate between the roles of Punnett square and Pedigree analysis 

Since both pedigrees and Punnett square are primarily used as genetic tools, students tend to get confused between their roles and utilization. Teachers can take an advent and explain the basic differences between them.

Pedigree: They are used when the inheritance of a gene is in the picture and being studied in depth. They represent the overall family history of any trait and how it gets inherited from parents to progeny within that family,

Punnett square: They are used to display all possible genotypes that a progeny can inherit from their parents. This is done by denoting all possible genotypes for a particular phenotype in all the generations, irrespective of the parents, offspring, siblings, etc.

punnett square diagram

Figure: A simple Punnett square. Image Source

3.Clarify using real-life examples

Educators are encouraged to use different types of Punnett Squares in their classes where students can see how genetics works.

Example: Use monohybrid, dihybrid, and trihybrid cross to explain the concept of independent assortment. While monohybrid crosses are used to understand the inheritance of a single allele only, dihybrid crosses are used to understand the inheritance of two different alleles at a time. When you show that a dihybrid cross can be solved by building two different monohybrid crosses from each trait, they can understand that the inheritance pattern of one allele doesn’t meddle and interfere with the independent inheritance of the other allele.

Similarly, many different Punnett squares can be built for autosomal diseases (dominant and recessive), and sex-linked diseases (dominant and recessive) and can be explained to students. This will widen their horizons about the utility of this tool.

4. Familiarize them with their practical use in genetic diagnosis

Students must be educated about the real-world applications of the Punnett squares when they are being trained to master this technique. This can be encouraging for them to learn it with more passion and enthusiasm. Punnett squares aren’t just academic games but find immense applications in the real world. We list some of them for you to begin with.

  • Used by genetic counselors as predictive tools for several genetic conditions

  • Used by genetic counselors as predictive tools for family planning 

Example: If your partner is a carrier for a genetically inherited disease (for example cystic fibrosis, color blindness, hemophilia), it is advisable to consult and check with your genetic counselors before conception. They would be able to predict the chance of your baby getting affected by solving the Punnett square and doing pedigree analysis. 

  • punnet square virtual lab

    Used to study genealogies for extensively studying human genetics. 

  • Used by plant breeders for developing higher-yielding varieties of plants.

  • Used by animal breeders to select superior parents for their farms.

  • Used to create useful databases for overall medical research.


Figure: Snippet from the Inheritance with Punnett squares simulation from Labster can be used to explain the concept of different states; homozygous and heterozygous. Full simulation is available for School and University/College classes.

5. Use virtual lab simulations

Punnett squares can be very complex to deal with in a single or handful number of classes. This is a topic that can be both difficult to assimilate for students and complex to deliver for educators. 

With a long list of complexities involved in this subject, it comes as a big challenge for educators at both high school and university/college levels. 

To deal with this issue, we at Labster, recommend modern-day educators and teachers use the Inheritance with Punnett squares simulation in their next classes. It can help you in delivering an effective session on the subject and related terminologies. Your students can actively learn about color blindness and eye color using the basic principles of inheritance and by virtually drawing a Punnett square. 

As they construct their virtual Punnett square, you can explain the idea of pure breeding in mice and emphasize the role of genes in dictating the mice’s fur color. Using the simulation, you can explain how the dominant allele can be identified by crossing multiple generations. 

The 3D simulations will help them better understand the intricacies of Punnett squares. Our interactive Inheritance with Punnett squares simulation along with gamification elements ensures that your students follow what you are trying to convey in the class. By using this way of active and immersive teaching, our virtual learning platform takes an advent in the field of Science to make the upcoming scientists thorough with the “basics of their respective subjects”. 

You can learn more about the Inheritance with Punnett squares simulation from Labster here or get in touch to find out how you can start using virtual labs with your students.

Figure: Snippet from the Inheritance with Punnett squares simulation from Labster used for finding the relation of mice’s fur color to genes using Punnett square. Full simulation is available for School and University/College classes.

Inheritance with Punnett Squares Virtual Lab