ChIP-exo
Perform the chromatin immunoprecipitation with exonuclease treatment (ChIP-exo) protocol, and prepare the target DNA to learn how E. coli survive in acid stress conditions.
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In the ChIP-exo simulation, you will perform the chromatin immunoprecipitation with exonuclease treatment (ChIP-exo) protocol in order to get a DNA sequence to elucidate the acid stress mechanism of Escherichia coli. You will learn how to fix the protein-DNA interactions and lyse the cells using a sonicator, add magnetic beads to the sample, and perform different on-bead and off-bead enzymatic modifications. From this, you will learn how the exonucleases work.
Will you be able to prepare the target DNA to understand how E. coli survive in acid stress conditions?
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