Time to complete course: 49 min.

About ChIP-exo Virtual Lab Simulation

In the ChIP-exo simulation, you will perform the chromatin immunoprecipitation with exonuclease treatment (ChIP-exo) protocol in order to get a DNA sequence to elucidate the acid stress mechanism of Escherichia coli. You will learn how to fix the protein-DNA interactions and lyse the cells using a sonicator, add magnetic beads to the sample, and perform different on-bead and off-bead enzymatic modifications. From this, you will learn how the exonucleases work.

Will you be able to prepare the target DNA to understand how E. coli survive in acid stress conditions?

Get Started Now

Perform the chromatin immunoprecipitation with exonuclease treatment (ChIP-exo) protocol, and prepare the target DNA to learn how E. coli survive in acid stress conditions.

Techniques In Lab

  • Cell lysis
  • Immunodetection
  • PCR
  • Sonication
  • Immunoprecipitation
  • DNA editing

 

Learning Objectives

At the end of this simulation, you will be able to…

  • Understand the use of exonuclease treatment in ChIP-exo technique.
  • Understand the key steps for performing the ChIP-exo technique as well as the importance of each reagent:
    • Adding formaldehyde to the culture to fix the bonds between proteins and DNA.
    • Lysing the cells using lysozyme and sonication.
    • Addition of specific antibodies.
    • Use of magnetic beads.
    • On-bead enzymatic modifications.
    • Off-bead enzymatic modifications.
  • Understand the molecular mechanism of ChIP-exo.

Screenshots of ChIP-exoVirtual Lab Simulation

Collaborators

How it works

A million dollar lab in your browser

Perform experiments in virtual lab simulations to achieve core science learning outcomes. 

All our simulations run on laptop and desktop computers, and you can play our simulations without having to install any browser plugins.

See detailed minimum requirements here.

Hundreds of hours of science learning content

Our virtual laboratory simulations are aimed at university, college and high school level, within fields such as biology, biochemistry, genetics, biotechnology, chemistry, physics and more.

With access to our simulations, you will have hundreds of hours of engaging, high-quality learning content available to you.

Discover more Virtual Lab Simulations

We currently have 146 simulations that cover everything from biology and medicine to physics and chemistry.

Learn how you can use Labster in your science course

IMPORTANT: Check the original email with instructions from your instructor to verify how you should gain access.

Link Access

Did you receive a link/URL to access Labster from your teacher? This is for Labster’s Quick Access, and no login is required. See more info below.

School LMS

Are you accessing Labster from your school’s LMS, such as Canvas, Blackboard, Moodle or Brightspace? Click below to read more.

Course Code

Did you receive a Course Code from your teacher? (this is different from a purchased voucher code. Learn how to access the Labster LMS, or log in below.
IMPORTANT: Please check your original email to confirm the correct access option.

Quick Access

Did you receive a link/URL to access the simulations through Labster’s Quick Access page? If so, no login is required. See more info below.

School LMS

Are you accessing Labster from your school’s LMS, such as Canvas, Blackboard, Moodle or Brightspace? Click below to read more.

Labster LMS

Are you accessing Labster directly from the Labster.com edX LMS? View more information here or click the button below to log in.

Faculty Resources

To access the Faculty Resources page (instructors only) and review Labster simulations, please log in below.