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About This Simulation
Let’s awaken your curiosity for the chemistry of caffeine. Learn how Flow Injection Analysis (FIA) helps scientists measure the concentration of caffeine in your drinks.
Learning Objectives
- Measure caffeine concentration in different samples using the Flow Injection Analysis (FIA) technique
- Describe the importance of the different parts of the FIA machine (e.g. the spectrophotometer, the pump, or the mixing coil)
- Describe the advantages of the FIA method
- Calculate the velocity at which a sample will be eluted
- Prepare sample dilutions and standard curves
- Interpret the results from a FIA experiment
- Determine the concentration of an unknown caffein sample by using the standard curve
About This Simulation
Lab Techniques
- Flow Injection Analysis
Related Standards
- HS-PS1-3, HS-PS2-6
- 3.9 Separation of Solutions and Mixtures Chromatography
- B.9 Biological Pigments
Learn More About This Simulation
Lego blocks and caffeine make an interesting combo. In this simulation, you will learn that a couple of scientists actually used lego blocks to build the first version of the Flow Injection Analysis (FIA) machine. You’ll use the technique to measure caffeine levels in various beverages.
Explore the parts of the flow injection machine
Not every real-life lab has a FIA machine, and it’s definitely not everyday that you get to look inside one. So in this simulation, you’ll get to explore the different parts of the FIA machine in detail through a 3D animation that illustrates the main mechanisms of the machine. You’ll soon understand how the machine detects caffeine, and apply this knowledge to your experiments.
Optimize and perform the experiment
Before you carry out an experiment, you will have to optimize the FIA machine for use. You can alter different machine parameters, to help generate the most accurate results. Once you have optimized the machine and prepared samples, you are ready to perform your experiment! Lots of beverages are waiting to be tested.
Compute the caffeine concentration
When you have finished your experiment, you’ll want to present your results in the best way possible. After creating an absorbance graph, you’ll employ your stoichiometry knowledge to determine the caffeine concentration of each beverage.
Maybe coffee is not the best drink for getting up or staying awake after all… maybe it’s hot chocolate! Will you be able to find out which beverages will cure you from sleepiness?
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North Dakota State College of Science
“They did the simulation at home, then completed the in-person lab within 30 minutes, no questions asked, and passed the quiz with flying colors.”
Lecturer in Human Physiology
University of Westminster
"I saw some of the students who clearly didn’t necessarily like sitting there reading a book discover they could turn on Labster and keep up with the rest of the class because it spoke to them.
Modesto City Schools
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Wenatchee Valley College
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San José State University
"We surveyed over 400 students. More than 90% thought Labster was easy to navigate, and that it was fun, but more importantly, most of them felt confident that they could execute the labs in person. And that confidence is a big deal."
FAQs
Find answers to frequently asked questions.
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A Labster virtual lab is an interactive, multimedia assignment that students access right from their computers. Many Labster virtual labs prepare students for success in college by introducing foundational knowledge using multimedia visualizations that make it easier to understand complex concepts. Other Labster virtual labs prepare learners for careers in STEM labs by giving them realistic practice on lab techniques and procedures.
Labster’s virtual lab simulations are created by scientists and designed to maximize engagement and interactivity. Unlike watching a video or reading a textbook, Labster virtual labs are interactive. To make progress, students must think critically and solve a real-world problem. We believe that learning by doing makes STEM stick.
Yes, Labster is compatible with all major LMS (Learning Management Systems) including Blackboard, Canvas, D2L, Moodle, and many others. Students can access Labster like any other assignment. If your institution does not choose an LMS integration, students will log into Labster’s Course Manager once they have an account created. Your institution will decide which is the best access method.
Labster is available for purchase by instructors, faculty, and administrators at education institutions. Purchasing our starter package, Labster Explorer, can be done using a credit card if you are located in the USA, Canada, or Mexico. If you are outside of North America or are choosing a higher plan, please speak with a Labster sales representative. Compare plans.
Labster supports a wide range of STEM courses at the high school, college, and university level across fields in biology, chemistry, physics, and health sciences. You can identify topics for your courses by searching our Content Catalog.