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Introductory Lab

Learn about lab safety, scientific methods, diffusion and osmosis, acids, bases and how to prepare buffers.

About This Simulation

In this experiment students will learn about acids and bases as well as proper risk evaluation in the laboratory. They will mix acid/base with water and watch a 3D animation showing how it dissociates to produce [H3O+] or [OH-]. Students will also learn how to calculate pH depending on if it is a strong acid/base or a weak acid/base. They are introduced to the concept of acid dissociation constant (Ka) and base dissociation constant (Kb).

Diffusion and osmosis are two fundamental concepts in biotechnology that are also important in daily life. In this experiment, students will understand the difference of diffusion and osmosis. They will observe under the microscope how cells adapt to hypotonic or hypertonic environments based on the principle of osmosis. Students will also learn that environments are dynamic, for example, cells that are already swollen can be reverted back to normal by adding hypertonic solution.

In this module, students will conduct a blood type test experiment. They are presented with a patient that is in desperate need of a blood transfusion. Students will learn about the different blood type groups (both ABO and Rh) and the consequences of incompatible blood transfusions. In the laboratory, students will use a typical blood type test card to determine the blood group. By observing the result, whether the sample clots or not, students will be able to draw a conclusion and determine the blood group.

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Collaborators

Learning Objectives

  • Learning about safety in the laboratory
  • Calculating pH of strong acids, weak acids, strong bases and weak bases
  • Understanding how acid dissociates in water
  • Understanding the principle of diffusion and osmosis
  • Understanding the different blood type groups and how to perform blood type test

Techniques

  • Blood typing
  • pH determination
  • Light microscopy

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