Kjeldahl Method: Estimate the protein content in food Virtual Lab

Time to complete course: 30 min.Need help? Visit our Help Center.

About the Kjeldahl Method: Estimate the protein content in food Virtual Lab Simulation

In the Kjeldahl Method simulation, you will learn how to use the Kjeldahl method to determine the protein content of a food sample, and how to use LC-MS/MS to investigate if the sample has been adulterated to make the protein content seem higher than it is.

Analyze a milk powder sample

Your lab has received batch samples of milk powder from 3 different producers. Your mission is to analyze a sample from one of the batches using the Kjeldahl method, in order to determine the exact protein content, and to make sure this is consistent with the reported value.

Tracing the steps of the Kjeldahl method

An animation will introduce you to each step of the analysis, and show what goes on at the molecular level.You will then perform the acid digestion, steam distillation and finally a colorimetric titration, before being challenged with the calculations needed to transfer the lab result to a protein content in the sample.

Is everything what it appears to be?

How can you be certain that your findings are correct? There might be ways to fool the Kjeldahl method! In the second half of the simulation, you will look into how this can happen, and explore the powerful technique LC-MS/MS, which can be used to detect if a known adulterant has been added to any of the received milk powder batches.

Will you be able to safely release the milk powder batches for consumers around the world?

Get Started Now

Measure the protein content in a food sample with the Kjeldahl method, and investigate whether the apparent result has been tampered with by using LC-MS/MS.

Techniques In Lab

  • Kjeldahl method
  • LC-MS/MS chromatogram interpretation

Learning Objectives

At the end of this simulation, you will be able to…

  • Describe the steps involved in the Kjeldahl method
  • Analyze the nitrogen content of a food sample
  • Calculate the protein content of a sample from the determined nitrogen content
  • Explain why the Kjeldahl method cannot be used to identify milk powder that was cut with melamine
  • Apply the principles of spiking and recovery in analytical chemistry
  • Interpret basic LC-MS/MS data
  • Describe the fundamentals of an LC-MS/MS analysis
  • Argue whether food fraud has been conducted based on available data

Screenshots of Kjeldahl Method: Estimate the protein content in food Virtual Lab Simulation

Collaborators

Dr. Melanie M. Erzinger

Dr. Melanie M. Erzinger

Laboratory of Food Biochemistry

ETH Zürich

Dr. Samy Boulos

Dr. Samy Boulos

Laboratory of Food Biochemistry

ETH Zürich

Sarah Frédérickx

Sarah Frédérickx

Dep. of Health Sciences and Technology

ETH Zürich

Dr. Laura Nyström

Dr. Laura Nyström

Dep. of Health Sciences and Technology

ETH Zürich

How it works

A million dollar lab in your browser

You can now perform advanced experiments to achieve core science learning outcomes. Theory and practise is connected in a novel way, and all you need is a computer/laptop and a browser!

Our simulations run even on older laptops and computers, and you will be able to play our simulations without having to install any browser plugins.

See detailed minimum requirements here.

100s of hours of science learning content

Explore many different science topics at your pace and difficulty level.

Our virtual laboratory simulations are aimed at university, college and high school level, within fields such as Biology, Biochemistry, Genetics, Biotechnology, Chemistry, Physics and many more.

With unlimited access to more than 60 different full simulations, you will have hundreds of hours of fun, innovative and high quality learning content available to you.

Study faster with the best award-winning science education tool

Learn anytime, anywhere.

Learn anytime, anywhere.

Labster is a 24/7 state-of-the-art virtual lab at your disposal. Study and learn at your own pace from anywhere.

Money back guarantee.

Money back guarantee.

Unlimited access to your own million dollar laboratory for just $19 per month. Full money back guarantee for your first paid month.

A million dollar lab on your laptop.

A million dollar lab on your laptop.

Turn your laptop into a lab-top with unlimited access to Labster’s fast-growing, immersive blended-learning experience. We offer 60+ simulations.

Trusted and used by top universities globally

What students think about Labster:

"A very innovative, immersive learning experience. It allows you to be more confident before going to the real laboratory. It's mind-blowing!"

Alice

Alice

Student Ambassador at Exeter Uni

"A great way to learn, because it can be very hard if you are just taught the theory - here you can try it yourself."

Ellie

Ellie

Student Ambassador at Exeter Uni

"More people should know about this! Some students don't really like science and this tool is amazing to get students passionate about science by making it fun and easy to learn."

Lucy

Lucy

Student Ambassador at Exeter Uni

FAQ

Frequently Asked Questions

I'm using Labster as part of my course. Do I need to buy a subscription?

If you are enrolled in a class and instructed by your teacher to use Labster, please make sure you use the sign-up information that has been provided by your teacher. Otherwise, your they will not be able to follow your progress and achievements, and you may need to purchase a license twice.

How many simulations will I get access to?

You will get unlimited access to all our simulations. We release new simulations nearly every month, and you can always see an overview of all our simulations in our simulation catalog.

When will my account renew?

Your subscription will renew automatically each month after the free trial is over, unless you have made a single simulation purchase.

How can I cancel my subscription?

If you would like to unsubscribe, please use this form. An automated confirmation email will be sent out to you in the next few minutes. Click the confirmation link contained in the email in order to finalize the termination of your subscription.

What are the minimum requirements for my computer?

You can find an overview of the minimum requirements here.