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About This Simulation
Gain insight into how scientists can improve children’s eyesight by genetically modifying E.coli to produce more beta-carotene.
Learning Objectives
- Explain the concept and molecular mechanism of MAGE technique
- Describe the oligo design requirements for MAGE
- Understand which proteins, enzymes, and plasmids are involved in MAGE
- Perform MAGE cycles
About This Simulation
Lab Techniques
- MAGE (Multiplex automated genome engineering)
- Electroporation
Related Standards
- No direct alignment
- No direct alignment
- No direct alignment
Learn More About This Simulation
Imagine you were tasked to edit over 100 genes to find which gene or combinations of genes will work best to produce the pigment beta-carotene. You could try editing them one at a time, but that would take way too much time in the lab! That’s where Multiplex Automated Genome Engineering, or MAGE, comes in. Instead of painstakingly editing one gene at a time, the MAGE technique helps scientists to perform many genetic mutations at many target sites at a time.
Introduction to MAGE and designing oligos
In this simulation, you will be introduced to the principles of MAGE as a recombinant engineering tool for the large-scale programming and accelerated evolution of cells. After understanding the basics of the technique, you will be tasked to perform MAGE to enhance the beta-carotene metabolic pathway in Escherichia coli. To start your MAGE journey, you will first learn how to design the optimum oligos to be used for editing E.Coli genomes.
Performing MAGE
Once you’ve understood the principles behind MAGE, you will have the freedom to experiment with changing up different parameters of the technique as you progress. For example, you can try tweaking cell density, growth media electroporation process, and MAGE cycle. Your decision will determine the outcome of your experiment!
Plating and screening
To help you visualize what happens at the molecular level in this technique, this simulation shows you the step-by-step progression of the MAGE cycle in immersive 3D animations. The choices that you made when designing your experiment at the beginning of the simulation will be portrayed in the screening steps of the resulting E.Coli clones.
Will you be able to enhance the beta-carotene production in E. coli to help improve the eyesight of young children?
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FAQs
Find answers to frequently asked questions.
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A Labster virtual lab is an interactive, multimedia assignment that students access right from their computers. Many Labster virtual labs prepare students for success in college by introducing foundational knowledge using multimedia visualizations that make it easier to understand complex concepts. Other Labster virtual labs prepare learners for careers in STEM labs by giving them realistic practice on lab techniques and procedures.
Labster’s virtual lab simulations are created by scientists and designed to maximize engagement and interactivity. Unlike watching a video or reading a textbook, Labster virtual labs are interactive. To make progress, students must think critically and solve a real-world problem. We believe that learning by doing makes STEM stick.
Yes, Labster is compatible with all major LMS (Learning Management Systems) including Blackboard, Canvas, D2L, Moodle, and many others. Students can access Labster like any other assignment. If your institution does not choose an LMS integration, students will log into Labster’s Course Manager once they have an account created. Your institution will decide which is the best access method.
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Labster supports a wide range of STEM courses at the high school, college, and university level across fields in biology, chemistry, physics, and health sciences. You can identify topics for your courses by searching our Content Catalog.