Multiplex Automated Genomic Engineering (MAGE): Conjuring massive mutations Virtual Lab

Gain insight into how scientists can improve children’s eyesight by genetically modifying E.coli to produce more beta-carotene.

  • University / College
Watch video
taylors_university
essex_university
ottawa_university
arizona_university

Labster is used by 1000s of amazing schools and universities

Learn more

About This Simulation

Imagine you were tasked to edit over 100 genes to find which gene or combinations of genes will work best to produce the pigment beta-carotene. You could try editing them one at a time, but that would take way too much time in the lab! That’s where Multiplex Automated Genome Engineering, or MAGE, comes in. Instead of painstakingly editing one gene at a time, the MAGE technique helps scientists to perform many genetic mutations at many target sites at a time.

Introduction to MAGE and designing oligos

In this simulation, you will be introduced to the principles of MAGE as a recombinant engineering tool for the large-scale programming and accelerated evolution of cells. After understanding the basics of the technique, you will be tasked to perform MAGE to enhance the beta-carotene metabolic pathway in Escherichia coli. To start your MAGE journey, you will first learn how to design the optimum oligos to be used for editing E.Coli genomes.

Performing MAGE

Once you’ve understood the principles behind MAGE, you will have the freedom to experiment with changing up different parameters of the technique as you progress. For example, you can try tweaking cell density, growth media electroporation process, and MAGE cycle. Your decision will determine the outcome of your experiment!

Plating and screening

To help you visualize what happens at the molecular level in this technique, this simulation shows you the step-by-step progression of the MAGE cycle in immersive 3D animations. The choices that you made when designing your experiment at the beginning of the simulation will be portrayed in the screening steps of the resulting E.Coli clones.

Will you be able to enhance the beta-carotene production in E. coli to help improve the eyesight of young children?

Explore Multiplex Automated Genomic Engineering (MAGE): Conjuring massive mutations Virtual Lab Simulation

MAG MAGE 1
MAG MAGE 2
MAG MAGE 3
MAG MAGE 4

How do virtual labs work?

Engage students in science through interactive learning scenarios. Simulate experiments, train lab techniques, and teach theory through visual experiences that enhance long-term learning outcomes.

  • 250+ Web-based simulations that can be played on laptops and tablets without installing any software

  • Teacher dashboard to automate grading and track student progress

  • Embedded quizzes to help students master science content

  • Library of learning resources, lab reports, videos, theory pages, graphics and more

Get started now!
You can explore and assign simulations to your students right away.

  • Access to over 250 Labster simulations for free.
  • Exclusive educator access to all of Labster Course Manager content.
  • 30 days for free, no credit card needed.
  • Invite your students to play simulations and get their feedback.
course manager

Integrate with your LMS

Labster integrates with all major LMS (Learning Management Systems) so that educators can use their gradebooks to track students’ performance data and students can keep a record of their work. Labster is compatible with Canvas, Blackboard, Moodle, Google Classroom, Schoology, Sakai, and Brightspace / D2L. It’s also possible to use Labster without an LMS.

Learn more
lms-blackboard-logo
lms-clever-logo
lms-canvas-logo
lms-moodle-logo
lms-google-logo
lms-sakai-logo
lms-brightspace-logo
lms-classlink-logo
lms-schoology-logo