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MAGE

In the Multiplex Automated Genome Engineering (MAGE) Simulation, you will learn how to edit the genome of Escherichia coli in order to enhance the beta-carotene metabolic pathway that was previously inserted.

About This Simulation

Multiplex automated genome engineering (MAGE) is a recombinant engineering tool for large-scale programming and accelerated evolution of cells. Before MAGE was introduced by George Church, scientists would painstakingly edit one gene at a time, examine the phenotype changes, and start the whole process again. MAGE is fast and efficient, cheap, and highly scalable. In addition, it allows researchers to edit specific or random sites in the genome and it also operates continuously in-vivo. MAGE's main limitation on the other hand lies on its portability. It has been exclusively used in E.coli so far.

In the Multiplex Automated Genome Engineering (MAGE) Simulation, you will learn how to edit the genome of Escherichia coli in order to enhance the beta-carotene metabolic pathway that was previously inserted. You will design the oligos according to the technique requirements, perform different MAGE cycles, and screen the resulting clones. Will you be able to enhance the beta-carotene metabolic pathway to increase its production in E. coli?

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Learning Objectives

  • Understanding the concept of the MAGE technique
  • Understanding the requirements to be met when designing oligos for MAGE
  • Learning which proteins, enzymes, and plasmids are involved in MAGE
  • Learning how to perform MAGE cycles
  • Understanding the molecular mechanism of MAGE

Techniques

  • MAGE
  • Bacteria plating
  • Electroporation

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