MAGE Virtual Lab

Time to complete course: 59 min.Need help? Visit our Help Center.

About the MAGE Virtual Lab Simulation

Multiplex automated genome engineering (MAGE) is a recombinant engineering tool for large-scale programming and accelerated evolution of cells. Before MAGE was introduced by George Church, scientists would painstakingly edit one gene at a time, examine the phenotype changes, and start the whole process again. MAGE is fast and efficient, cheap, and highly scalable. In addition, it allows researchers to edit specific or random sites in the genome and it also operates continuously in vivo. MAGE’s main limitation on the other hand lies on its portability. It has been exclusively used in E.coli so far.

In the Multiplex Automated Genome Engineering (MAGE) lab, you will learn how to edit the genome of Escherichia coli in order to enhance the beta-carotene metabolic pathway that was previously inserted. You will design the oligos according to the technique requirements, perform different MAGE cycles, and screen the resulting clones. Will you be able to enhance the beta-carotene metabolic pathway to increase its production in E. coli?

Get Started Now

Learn how to edit the genome of Escherichia coli in order to enhance the beta-carotene metabolic pathway that was previously inserted.

Techniques In Lab

  • MAGE
  • Bacteria plating
  • Electroporation

Learning Objectives

At the end of this simulation, you will be able to…

  • Understand the concept of the MAGE technique
  • Understand the requirements to be met when designing oligos for MAGE
  • Understand which proteins, enzymes, and plasmids are involved in MAGE
  • Perform MAGE cycles
  • Understand the molecular mechanism of MAGE

Screenshots of MAGE Virtual Lab Simulation

Collaborators

How it works

A million dollar lab in your browser

You can now perform advanced experiments to achieve core science learning outcomes. Theory and practice is connected in a novel way, and all you need is a computer/laptop and a browser!

Our simulations run even on older laptops and computers, and you will be able to play our simulations without having to install any browser plugins.

See detailed minimum requirements here.

100s of hours of science learning content

Explore many different science topics at your pace and difficulty level.

Our virtual laboratory simulations are aimed at university, college and high school level, within fields such as Biology, Biochemistry, Genetics, Biotechnology, Chemistry, Physics and many more.

With unlimited access to more than 60 different full simulations, you will have hundreds of hours of fun, innovative and high quality learning content available to you.

Study faster with the best award-winning science education tool

Learn anytime, anywhere.

Learn anytime, anywhere.

Labster is a 24/7 state-of-the-art virtual lab at your disposal. Study and learn at your own pace from anywhere.

Money back guarantee.

Money back guarantee.

Unlimited access to your own million dollar laboratory for just $19 per month. Full money back guarantee for your first paid month.

A million dollar lab on your laptop.

A million dollar lab on your laptop.

Turn your laptop into a lab-top with unlimited access to Labster’s fast-growing, immersive blended-learning experience. We offer 60+ simulations.

Trusted and used by top universities globally

What students think about Labster:

"A very innovative, immersive learning experience. It allows you to be more confident before going to the real laboratory. It's mind-blowing!"

Alice

Alice

Student Ambassador at Exeter Uni

"A great way to learn, because it can be very hard if you are just taught the theory - here you can try it yourself."

Ellie

Ellie

Student Ambassador at Exeter Uni

"More people should know about this! Some students don't really like science and this tool is amazing to get students passionate about science by making it fun and easy to learn."

Lucy

Lucy

Student Ambassador at Exeter Uni

FAQ

Frequently Asked Questions

I'm using Labster as part of my course. Do I need to buy a subscription?

If you are enrolled in a class and instructed by your teacher to use Labster, please make sure you use the sign-up information that has been provided by your teacher. Otherwise, they will not be able to follow your progress and achievements, and you may need to purchase a license twice.

How many simulations will I get access to?

You will get unlimited access to all our simulations. We release new simulations nearly every month, and you can always see an overview of all our simulations in our simulation catalog.

When will my account renew?

Your subscription will renew automatically each month after the free trial is over, unless you have made a single simulation purchase.

How can I cancel my subscription?

If you would like to unsubscribe, please use this form. An automated confirmation email will be sent out to you in the next few minutes. Click the confirmation link contained in the email in order to finalize the termination of your subscription.

What are the minimum requirements for my computer?

You can find an overview of the minimum requirements here.