Select Page
MAGE
In the Multiplex Automated Genome Engineering (MAGE) Simulation, you will learn how to edit the genome of Escherichia coli in order to enhance the beta-carotene metabolic pathway that was previously inserted.

About This Simulation

In the Multiplex Automated Genome Engineering (MAGE) Simulation, you will learn how to edit the genome of Escherichia coli in order to enhance the beta-carotene metabolic pathway that was previously inserted. You will design the oligos according to the technique requirements, perform different MAGE cycles, and screen the resulting clones. Will you be able to enhance the beta-carotene metabolic pathway to increase its production in E. coli?

Screenshots

Collaborators

Learning Objectives

  • Understanding the concept of the MAGE technique
  • Understanding the requirements to be met when designing oligos for MAGE
  • Learning which proteins, enzymes, and plasmids are involved in MAGE
  • Learning how to perform MAGE cycles
  • Understanding the molecular mechanism of MAGE

Techniques

  • MAGE
  • Bacteria plating
  • Electroporation

Collaborator

List of all our learning simulations

View All Simulations