Multiplex Automated Genomic Engineering (MAGE): Conjuring massive mutations

MAGE
Time to complete course: 38 min.

About Multiplex Automated Genomic Engineering (MAGE): Conjuring massive mutations Virtual Lab Simulation

Imagine you were tasked to edit over 100 genes to find which gene or combinations of genes will work best to produce the pigment beta-carotene. You could try editing them one at a time, but that would take way too much time in the lab! That’s where Multiplex Automated Genome Engineering, or MAGE, comes in. Instead of painstakingly editing one gene at a time, the MAGE technique helps scientists to perform many genetic mutations at many target sites at a time.

Introduction to MAGE and designing oligos

In this simulation, you will be introduced to the principles of MAGE as a recombinant engineering tool for the large-scale programming and accelerated evolution of cells. After understanding the basics of the technique, you will be tasked to perform MAGE to enhance the beta-carotene metabolic pathway in Escherichia coli.  To start your MAGE journey, you will first learn how to design the optimum oligos to be used for editing E.Coli genomes.

Performing MAGE

Once you’ve understood the principles behind MAGE, you will have the freedom to experiment with changing up different parameters of the technique as you progress. For example, you can try tweaking cell density, growth media electroporation process, and MAGE cycle. Your decision will determine the outcome of your experiment!

Plating and screening

To help you visualize what happens at the molecular level in this technique, this simulation shows you the step-by-step progression of the MAGE cycle in immersive 3D animations. The choices that you made when designing your experiment at the beginning of the simulation will be portrayed in the screening steps of the resulting E.Coli clones. 

Will you be able to enhance the beta-carotene production in E. coli to help improve the eyesight of young children?

Get Started Now

Gain insight into how scientists can improve children’s eyesight by genetically modifying E.coli to produce more beta-carotene.

Techniques In Lab

  • MAGE (Multiplex Automated Genome Engineering)
  • Electroporation

Learning Objectives

At the end of this simulation, you will be able to…

  • Explain the concept and molecular mechanism of MAGE technique
  • Describe the oligo design  requirements for MAGE
  • Understand which proteins, enzymes, and plasmids are involved in MAGE
  • Perform MAGE cycles

Screenshots of Multiplex Automated Genomic Engineering (MAGE): Conjuring massive mutationsVirtual Lab Simulation

Collaborators

Anne Lykke

Novo Nordisk Foundation Center for Biosustainability

annly@biosustain.dtu.dk

Michael Schantz Klausen

Novo Nordisk Foundation Center for Biosustainability

mskl@biosustain.dtu.dk

How it works

A million dollar lab in your browser

Perform experiments in virtual lab simulations to achieve core science learning outcomes. 

All our simulations run on laptop and desktop computers, and you can play our simulations without having to install any browser plugins.

See detailed minimum requirements here.

Hundreds of hours of science learning content

Our virtual laboratory simulations are aimed at university, college and high school level, within fields such as biology, biochemistry, genetics, biotechnology, chemistry, physics and more.

With access to our simulations, you will have hundreds of hours of engaging, high-quality learning content available to you.

Learn how you can use Labster in your science course

The simulation is already added to this package

Step 1: Choose your simulations

Find and select the simulations that you want to add to your course.

Step 2: Review simulation list

This is the list of simulations that will be added to your course. Click Download once you are ready.

%%%selected_title%%%
No simulations added yet.
    %%%selected_title%%%
    Download the .zip file and upload it to your LMS.

    Download Common Cartridge File

    Choose your Learning Management System below:
    Generating download link. Please wait...
    Congratulations! You can now upload the .zip file into your LMS by the following these instructions:

    Simulation name

    Image container

    Learning Outcomes

    Learning outcomes container

    Techniques

    Techniques container

    Simulation Description

    Description container

    Are you sure you want to delete?

    Package deleted successfully

    IMPORTANT: Check the original email with instructions from your instructor to verify how you should gain access.

    Link Access

    Did you receive a link/URL to access Labster from your teacher? This is for either “Labster Direct” or “Quick Access”, and no login is required. See info below.

    School LMS

    Are you accessing Labster from your school’s LMS, such as Canvas, Blackboard, Moodle or Brightspace? Click below to read more.

    Course Code

    Did you receive a Course Code from your teacher? (this is different from a purchased voucher code. Learn how to access the Labster LMS, or log in below.
    IMPORTANT: Please check your original email to confirm the correct access option.

    Labster Direct / Quick Access

    Did you receive a link/URL to access simulations through the Labster Direct or Quick Access pages? If so, no login is required. See info below.

    Your LMS

    Are you accessing Labster from your school’s LMS, such as Canvas, Blackboard, Moodle or Brightspace? Click below to read more.

    edX

    Are you accessing Labster directly from the Labster.com edX LMS? View more information here or click the button below to log in.

    Faculty Resources

    To access the Faculty Resources page (instructors only) and review Labster simulations, please log in below.
    Cookie Box Settings