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Cancer Sample Preparation for Mass Spectrometry | Virtual Lab

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Higher Education
 
Cancer Sample Preparation for Mass Spectrometry
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About This Simulation

Assist in a biopsy on a cancer patient and learn how to prepare the sample for mass spectrometry by extracting its phosphopeptides. Will you be able to identify the cancer stage of the patient by looking at the phosphorylation pattern?

Learning Objectives

  • Understand the correlation between phosphorylation and cancer
  • Prepare a biopsy sample for mass spectrometry

About This Simulation

Level:
Higher Education
Length:
44
Min
Accessibility Mode:
Available
Languages:
English

Lab Techniques

  • Phosphopeptide enrichment
  • Protein purification
  • Enzymatic digestion
  • Cell lysis
No lab techniques are listed for this simulation.

Related Standards

University:
NGSS:
  • No direct alignment
AP:
  • No direct alignment
LB:
  • No direct alignment
No lab techniques are listed for this simulation.

Learn More About This Simulation

Take action! Cancer is on the rise, and you can help with diagnosis and treatment. In the Cancer Sample Preparation for Mass Spectrometry simulation, you will assist doctors by determining the cancer stage of a patient diagnosed with colon cancer. This is the first simulation in the Mass Spectrometry series.

Prepare the biopsy

Join doctors in the hospital and enter an immersive animation to see how we perform a colorectal cancer biopsy. Once back in the lab, your mission will be to extract proteins from the tumor sample. Find the equipment you need in the lab to lyse the tumor cells, denature the proteins, and perform phosphopeptide enrichment, in order to prepare the sample for mass spectrometry.

Explore the molecules

Use the holotable to observe detailed 3D models of the molecules involved in phosphorylation. Get an understanding of the molecular principles underlying the biopsy preparation and how the phosphorylation pattern relates to cancer.

Interpret your findings

In the end, you will analyze the results from the mass spectrometry on a graph. Will you be able to help guide the patient’s treatment by identifying their cancer stage?

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