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Microscopy Virtual Lab

Analyze the microscopic structure of the small intestine and learn the advantages and limitations of light, fluorescence and electron microscopy.

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Microscopy

About this Simulation

Join the Microscopy lab and learn about the different types of microscopy to understand the mechanisms behind it. You will be trained in light microscopy, transmission electron microscopy and fluorescence microscopy.

Use magnification

In the Microscopy lab, you will be presented with chicken intestinal slides that have been stained with Anilin, Orange G and Fuchsin. Using the 5x magnification, you will identify the villus and then proceed with higher magnifications to identify smooth muscle, Relatedcellular tissue, epithelial cells, Goblet cells and the nuclei.

Try out the electron microscope

Electron microscopes can be used to visualize objects that are too small to see when using a light microscope—for example, the microvilli, mitochondria and the junctions between cells. In the Microscopy lab, you will examine a chicken intestine slide that is specially prepared for a transmission electron microscope. You can zoom in and out to observe different cellular structures.

Learn about fluorescence staining techniques

You will learn about fluorescence staining techniques and how they can be used to visualize specific structures. For example, by staining the DNA with DAPI, you can easily identify a cell’s nucleus. In this part of the lab, you will examine a chicken intestine sample that is infected with a retrovirus and observe how the virus infects the lymphocytes and how it inhibits inflammation. The retrovirus can be further developed as a medicine for coeliac disease.

Learning Objectives

At the end of this simulation, you will be able to:

  • Understand different microscopy techniques and their limitations

  • Identify various cell types and cellular structures

  • Understand coeliac disease and intestinal inflammation

  • Understand staining techniques

Techniques in Lab

At the end of this simulation, you will be able to:

  • Fluorescence Microscopy
  • Light microscopy: using immersion oil and working with different objectives

Explore:

Microscopy

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