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About Microscopy Virtual Lab Simulation
This is the original, full-length “Microscopy” simulation. Please see the newer “Light Microscopy” and “Fluorescence Microscopy” simulations.
Join the Microscopy lab and learn about the different types of microscopy to understand the mechanisms behind. You will be trained in light microscopy, transmission electron microscopy and fluorescence microscopy.
Use magnification
In the Microscopy lab, you will be presented with chicken intestinal slides that have been stained with Anilin, Orange G and Fuchsin. Using the 5x magnification, you will identify the villus, and then proceed with higher magnifications to identify smooth muscle, extracellular tissue, epithelial cells, Goblet cells and the nuclei.
Try out the electron microscope
Electron microscopes can be used to visualize objects that are too small to see when using a light microscope—for example the microvilli, mitochondria and the junctions between cells. In the Microscopy lab, you will examine a chicken intestine slide that is specially prepared for a transmission electron microscope. You can zoom in and out to observe different cellular structures.
Learn about fluorescence staining techniques
You will learn about fluorescence staining techniques and how it can be used to visualize specific structures. For example, by staining the DNA with DAPI, you can easily identify a cell’s nucleus. In this part of the lab, you will examine a chicken intestine sample that is infected with a retrovirus and observe how the virus infects the lymphocytes and how it inhibits inflammation. The retrovirus can be further developed as medicine for coeliac disease.
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Techniques in Lab
- Light microscopy: using immersion oil and working with different objectives
- Fluorescent microscopy
Learning Objectives
At the end of this simulation, you will be able to…
- Understand different microscopy techniques and their limitations
- Identify various cell types and cellular structures
- Understand coeliac disease and intestinal inflammation
- Understand staining techniques
Simulation Features
Examples of Related Standards
Life Science related 1st year onwards
Not directly assessed, could support many areas depending on educators approach
1.1 Introduction to cells*
1.2 Ultrastructure of cells
Unit 2: Cell Structure and Function
Screenshots of Microscopy Virtual Lab Simulation
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