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Pipetting: Master the technique | Virtual Lab

Higher Education
Health Sciences
Biology
Chemistry
Pipetting: Master the technique
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About This Simulation

Micropipettes are the extended arm of every biochemist. In this simulation, you will learn the art of pipetting in a hands-on experiment, as you try to determine the protein content of a corn cob. Will you be able to help LabsterFood Inc. create a breed of corn that contains enough essential amino acids to fulfil a person’s daily requirements? After playing this simulation, you’ll be ready to show off your pipetting skills when you enter the lab for the first time.

Learning Objectives

  • Select the correct micropipette for its purpose
  • Use the two stops of the pipette
  • Explain pipetting techniques
  • Perform a serial dilution
  • Quantify the protein content in a sample with a Bradford assay

About This Simulation

Level:
Higher Education
Length:
34
Min
Accessibility Mode:
Available
Languages:
English
German
Spanish
French
Italian

Lab Techniques

  • Serial dilution
  • Bradford assay
  • Pipetting
No lab techniques are listed for this simulation.

Related Standards

University:
  • Early Stage Bachelors Level
  • EHEA First Cycle
  • EHEA Short Cycle
  • FHEQ 4
  • FHEQ 5
  • FHEQ 6
  • Support for laboratory skills at all levels
  • US College Year 2
  • US College Year 1
NGSS:
  • Generic support for fume hood use, lab safety and good scientific practice
AP:
  • Generic support for relevant Lab Investigations
LB:
  • Generic technical support for practical skill development
No lab techniques are listed for this simulation.

Learn More About This Simulation

In the Pipetting simulation, you will determine the amount of essential amino acids in genetically modified corn. Using a micropipette, you will prepare a serial dilution, and measure the protein concentration with a Bradford assay.

Get to know the micropipette

You will be introduced to the basics of pipetting as you dive right into the experiments. Step by step, you will learn how to adjust the volume, use the two ‘stops’ of the pipette, and find out which pipette to use for which volume range. You will also learn tips and tricks to make your pipetting more accurate.

Perform a serial dilution

Once you master the art of pipetting, you will dilute your protein solution 10.000 fold! While you progress through the lab, you will become more and more routined and independent. After this mission, you will understand the benefits of serial dilutions, and be able to perform one by yourself.

Measure the protein content in the sample

In this mission, you will dye your proteins blue, and use your new skills to prepare a microplate for a Bradford assay. In this colorimetric assay, you shine light of a specific wavelength through the plate, and measure the absorbance in each well. Finally, you will compare the absorbances of your sample to a sample of known protein concentration.

Will you be able to calculate the amount of essential amino acids in the corn cob you collected?

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Professor Margaret Brady was able to enhance student learning with A&P virtual labs.
Margaret Brady
Associate Professor
North Dakota State College of Science

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PhD
Lecturer in Human Physiology
University of Westminster

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Curriculum Coordinator
Modesto City Schools

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Adjunct Instructor
Wenatchee Valley College

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Chemistry Lecturer
San José State University

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Lecturer
Northumbria University

“The Labster simulations get students to do things, and they're not just sitting there consuming a webinar where their mind can drift. They become an active participant in that learning experience.”

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