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About This Simulation
Micropipettes are the extended arm of every biochemist. In this simulation, you will learn the art of pipetting in a hands-on experiment, as you try to determine the protein content of a corn cob. Will you be able to help LabsterFood Inc. create a breed of corn that contains enough essential amino acids to fulfil a person’s daily requirements? After playing this simulation, you’ll be ready to show off your pipetting skills when you enter the lab for the first time.
Learning Objectives
- Select the correct micropipette for its purpose
- Use the two stops of the pipette
- Explain pipetting techniques
- Perform a serial dilution
- Quantify the protein content in a sample with a Bradford assay
About This Simulation
Lab Techniques
- Serial dilution
- Bradford assay
- Pipetting
Related Standards
- Early Stage Bachelors Level
- EHEA First Cycle
- EHEA Short Cycle
- FHEQ 4
- FHEQ 5
- FHEQ 6
- Support for laboratory skills at all levels
- US College Year 2
- US College Year 1
- Generic support for fume hood use, lab safety and good scientific practice
- Generic support for relevant Lab Investigations
- Generic technical support for practical skill development
Learn More About This Simulation
In the Pipetting simulation, you will determine the amount of essential amino acids in genetically modified corn. Using a micropipette, you will prepare a serial dilution, and measure the protein concentration with a Bradford assay.
Get to know the micropipette
You will be introduced to the basics of pipetting as you dive right into the experiments. Step by step, you will learn how to adjust the volume, use the two ‘stops’ of the pipette, and find out which pipette to use for which volume range. You will also learn tips and tricks to make your pipetting more accurate.
Perform a serial dilution
Once you master the art of pipetting, you will dilute your protein solution 10.000 fold! While you progress through the lab, you will become more and more routined and independent. After this mission, you will understand the benefits of serial dilutions, and be able to perform one by yourself.
Measure the protein content in the sample
In this mission, you will dye your proteins blue, and use your new skills to prepare a microplate for a Bradford assay. In this colorimetric assay, you shine light of a specific wavelength through the plate, and measure the absorbance in each well. Finally, you will compare the absorbances of your sample to a sample of known protein concentration.
Will you be able to calculate the amount of essential amino acids in the corn cob you collected?
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For Science Programs Providing a Learning Advantage
North Dakota State College of Science
“They did the simulation at home, then completed the in-person lab within 30 minutes, no questions asked, and passed the quiz with flying colors.”
Lecturer in Human Physiology
University of Westminster
"I saw some of the students who clearly didn’t necessarily like sitting there reading a book discover they could turn on Labster and keep up with the rest of the class because it spoke to them.
Modesto City Schools
"Having something that's engaging for the students gives teachers that opportunity to breathe and get excited again. Because they're seeing the kids light up, they're seeing the kids engage with content."
Wenatchee Valley College
"The question always is, ‘Can we demonstrate that the students are meeting course outcomes?’ Check! We can do that.”
San José State University
"We surveyed over 400 students. More than 90% thought Labster was easy to navigate, and that it was fun, but more importantly, most of them felt confident that they could execute the labs in person. And that confidence is a big deal."
FAQs
Find answers to frequently asked questions.
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A Labster virtual lab is an interactive, multimedia assignment that students access right from their computers. Many Labster virtual labs prepare students for success in college by introducing foundational knowledge using multimedia visualizations that make it easier to understand complex concepts. Other Labster virtual labs prepare learners for careers in STEM labs by giving them realistic practice on lab techniques and procedures.
Labster’s virtual lab simulations are created by scientists and designed to maximize engagement and interactivity. Unlike watching a video or reading a textbook, Labster virtual labs are interactive. To make progress, students must think critically and solve a real-world problem. We believe that learning by doing makes STEM stick.
Yes, Labster is compatible with all major LMS (Learning Management Systems) including Blackboard, Canvas, D2L, Moodle, and many others. Students can access Labster like any other assignment. If your institution does not choose an LMS integration, students will log into Labster’s Course Manager once they have an account created. Your institution will decide which is the best access method.
Labster is available for purchase by instructors, faculty, and administrators at education institutions. Purchasing our starter package, Labster Explorer, can be done using a credit card if you are located in the USA, Canada, or Mexico. If you are outside of North America or are choosing a higher plan, please speak with a Labster sales representative. Compare plans.
Labster supports a wide range of STEM courses at the high school, college, and university level across fields in biology, chemistry, physics, and health sciences. You can identify topics for your courses by searching our Content Catalog.