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Pipetting: Master the technique Virtual Lab

Micropipettes are the extended arm of every biochemist. In this simulation, you will learn the art of pipetting in a hands-on experiment, as you try to determine the protein content of a corn cob. Will you be able to help LabsterFood Inc. create a breed of corn that contains enough essential amino acids to fulfil a person’s daily requirements? After playing this simulation, you’ll be ready to show off your pipetting skills when you enter the lab for the first time.

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Pipetting: Master the technique

About this Simulation

In the Pipetting simulation, you will determine the amount of essential amino acids in genetically modified corn. Using a micropipette, you will prepare a serial dilution, and measure the protein concentration with a Bradford assay.

Get to know the micropipette

You will be introduced to the basics of pipetting as you dive right into the experiments. Step by step, you will learn how to adjust the volume, use the two ‘stops’ of the pipette, and find out which pipette to use for which volume range. You will also learn tips and tricks to make your pipetting more accurate.

Perform a serial dilution

Once you master the art of pipetting, you will dilute your protein solution 10.000 fold! While you progress through the lab, you will become more and more routined and independent. After this mission, you will understand the benefits of serial dilutions, and be able to perform one by yourself.

Measure the protein content in the sample

In this mission, you will dye your proteins blue, and use your new skills to prepare a microplate for a Bradford assay. In this colorimetric assay, you shine light of a specific wavelength through the plate, and measure the absorbance in each well. Finally, you will compare the absorbances of your sample to a sample of known protein concentration.

Will you be able to calculate the amount of essential amino acids in the corn cob you collected?

Learning Objectives

At the end of this simulation, you will be able to:

  • Select the correct micropipette for its purpose

  • Use the two stops of the pipette

  • Explain pipetting techniques

  • Perform a serial dilution

  • Quantify the protein content in a sample with a Bradford assay

Techniques in Lab

At the end of this simulation, you will be able to:

  • Serial dilution
  • Bradford assay
  • Pipetting

Explore:

Pipetting: Master the technique

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