About This Simulation
In the first mission, students will learn the difference about protein synthesis in prokaryote (using E. coli) and eukaryote (using CHO cells). The virtual lab starts by asking the students to prepare recombinant Erythropoietin that is transfected into E. coli and CHO cells. The lab assistant will prepare the recombinant EPO and students would be able to measure the mass to charge ratio using mass spectrometer. Students would be able to understand the basic principles of mass spectrometry by watching an introductory video in the labpad.
Students will also learn about the translation process from mRNA to amino acids and how amino acids are assembled to proteins. A 3D animation is shown describing how triplets of codons are translated into amino acids, how these amino acids are joined together by peptide bonds creating a primary structure of protein and further more how the primary structure is folded into secondary, tertiary and quaternary structure.
In the last mission, students need to use mass spectrometry and investigate if there are any athletes who are using rhEPO as a doping substance. They will do so by collaborating with the doping agent who collects urine sample in a large bicycle race. Will they be able to find the cheating athlete?