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About This Simulation
Investigate each step of SDS-PAGE from gel selection and sample preparation to chamber assembly and what really happens when the current turns on, to separate proteins solely by molecular weight, bringing us one step closer to identifying the protein.
Learning Objectives
- Assemble the SDS-PAGE apparatus including loading samples and size markers
- Select an appropriate gel and associated electrical settings for resolving the proteins of interest
- Critique the quality of a complete SDS-PAGE run using a removable protein stain
About This Simulation
Lab Techniques
- Gel electrophoresis
- SDS-PAGE
Related Standards
- Early Stage Bachelors Level
- Intermediate Stage Bachelors Level
- Late Stage Bachelors Level
- EHEA First Cycle
- US College Year 1
- US College Year 2
- US College Year 3
- US College Year 4
- Biology Unit 1.3: Introduction to Biological Macromolecules
- Biology 2.4
Learn More About This Simulation
SDS-PAGE creates conditions for separating proteins by an identifying characteristic, molecular weight. In this simulation, you will learn how SDS, the acrylamide gel, buffer solutions, and the electric current work together to separate proteins.
Running an SDS-PAGE
In this lab, students will determine if a particular sized protein is present in their samples. To do this, they must learn about the mechanisms of how SDS-PAGE works including deciding on which gel size to use, how the sample buffer prepares the sample, how to assemble the electrophoresis chamber, and ultimately the interactions between pH, the buffer solutions, and the electric current.
Mechanisms behind SDS-PAGE
In order to determine if the sample contains a protein of a specific molecular weight, students first need to select the right acrylamide gel concentration. This simulation allows them to trial different concentrations to see the effect on protein separation. From here, they prepare the sample through visualizing the effects of each component of the sample buffer on the sample itself. They will then run an SDS-PAGE by assembling the electrophoresis chamber and learning how the current and ions of the buffer solutions interact. With the results of the SDS-PAGE being ready immediately, students will determine which samples contain the target protein.
Gel Review
With SDS-PAGE thoroughly explored, students will evaluate their stained gel to identify if the samples have evidence of the target protein. They will also evaluate other gels as usable or unusable and identify reasons that may have caused the outcome.
By understanding SDS-PAGE, will you find the target protein and be able to identify a successful gel?
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For Science Programs Providing a Learning Advantage
North Dakota State College of Science
“They did the simulation at home, then completed the in-person lab within 30 minutes, no questions asked, and passed the quiz with flying colors.”
Lecturer in Human Physiology
University of Westminster
"I saw some of the students who clearly didn’t necessarily like sitting there reading a book discover they could turn on Labster and keep up with the rest of the class because it spoke to them.
Modesto City Schools
"Having something that's engaging for the students gives teachers that opportunity to breathe and get excited again. Because they're seeing the kids light up, they're seeing the kids engage with content."
Wenatchee Valley College
"The question always is, ‘Can we demonstrate that the students are meeting course outcomes?’ Check! We can do that.”
San José State University
"We surveyed over 400 students. More than 90% thought Labster was easy to navigate, and that it was fun, but more importantly, most of them felt confident that they could execute the labs in person. And that confidence is a big deal."
FAQs
Find answers to frequently asked questions.
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A Labster virtual lab is an interactive, multimedia assignment that students access right from their computers. Many Labster virtual labs prepare students for success in college by introducing foundational knowledge using multimedia visualizations that make it easier to understand complex concepts. Other Labster virtual labs prepare learners for careers in STEM labs by giving them realistic practice on lab techniques and procedures.
Labster’s virtual lab simulations are created by scientists and designed to maximize engagement and interactivity. Unlike watching a video or reading a textbook, Labster virtual labs are interactive. To make progress, students must think critically and solve a real-world problem. We believe that learning by doing makes STEM stick.
Yes, Labster is compatible with all major LMS (Learning Management Systems) including Blackboard, Canvas, D2L, Moodle, and many others. Students can access Labster like any other assignment. If your institution does not choose an LMS integration, students will log into Labster’s Course Manager once they have an account created. Your institution will decide which is the best access method.
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Labster supports a wide range of STEM courses at the high school, college, and university level across fields in biology, chemistry, physics, and health sciences. You can identify topics for your courses by searching our Content Catalog.