SDS-PAGE: Separating proteins by molecular weight
Investigate each step of SDS-PAGE from gel selection and sample preparation to chamber assembly and what really happens when the current turns on, to separate proteins solely by molecular weight, bringing us one step closer to identifying the protein.
- University / College





Labster is used by 1000s of amazing schools and universities
Learn moreAbout This Simulation
SDS-PAGE creates conditions for separating proteins by an identifying characteristic, molecular weight. In this simulation, you will learn how SDS, the acrylamide gel, buffer solutions, and the electric current work together to separate proteins.
Running an SDS-PAGE
In this lab, students will determine if a particular sized protein is present in their samples. To do this, they must learn about the mechanisms of how SDS-PAGE works including deciding on which gel size to use, how the sample buffer prepares the sample, how to assemble the electrophoresis chamber, and ultimately the interactions between pH, the buffer solutions, and the electric current.
Mechanisms behind SDS-PAGE
In order to determine if the sample contains a protein of a specific molecular weight, students first need to select the right acrylamide gel concentration. This simulation allows them to trial different concentrations to see the effect on protein separation. From here, they prepare the sample through visualizing the effects of each component of the sample buffer on the sample itself. They will then run an SDS-PAGE by assembling the electrophoresis chamber and learning how the current and ions of the buffer solutions interact. With the results of the SDS-PAGE being ready immediately, students will determine which samples contain the target protein.
Gel Review
With SDS-PAGE thoroughly explored, students will evaluate their stained gel to identify if the samples have evidence of the target protein. They will also evaluate other gels as usable or unusable and identify reasons that may have caused the outcome.
By understanding SDS-PAGE, will you find the target protein and be able to identify a successful gel?
Explore SDS-PAGE: Separating proteins by molecular weight Virtual Lab Simulation
How do virtual labs work?
Engage students in science through interactive learning scenarios. Simulate experiments, train lab techniques, and teach theory through visual experiences that enhance long-term learning outcomes.
250+ Web-based simulations that can be played on laptops and tablets without installing any software
Teacher dashboard to automate grading and track student progress
Embedded quizzes to help students master science content
Library of learning resources, lab reports, videos, theory pages, graphics and more
Get started now!
You can explore and assign simulations to your students right away.
- Access to over 250 Labster simulations for free.
- Exclusive educator access to all of Labster Course Manager content.
- 30 days for free, no credit card needed.
- Invite your students to play simulations and get their feedback.



Integrate with your LMS
Labster integrates with all major LMS (Learning Management Systems) so that educators can use their gradebooks to track students’ performance data and students can keep a record of their work.








