Select Page

USER

In this simulation, you will understand the principles of the Uracil-excision based cloning. Will you be able to insert the genes correctly into the desired plasmid?

About This Simulation

In this simulation, you will understand the principles of the Uracil-excision based cloning. You will design specific primers, perform the PCR, and use specific reagents to clone three genes of the Beta-carotene production pathway into an Escherichia coli strain, in order to start the compound’s production in a cell factory. In addition, you will also learn how to solve some common issues that can arise when using this technique. Will you be able to insert the genes correctly into the desired plasmid?

Screenshots

Collaborators

Learning Objectives

  • Understanding the principles of USER cloning
  • Learning how to design primers for USER cloning
  • Understanding the molecular mechanism behind the USER cloning
  • Understanding the critical points to design a USER cloning experiment

 

Techniques

  • USER

 

 

Collaborator

List of all our learning simulations