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About This Simulation
Join Marie in the molecular biology lab to apply your knowledge of the sophisticated cloning method, USER, in order to generate a beta-carotene cell factory.
Learning Objectives
- Understand the principles of USER cloning
- Design primers for USER cloning
- Describe the molecular mechanism behind the USER cloning
- Explain the critical points to design a USER cloning experiment
About This Simulation
Lab Techniques
- Uracil based excision cloning (USER)
Related Standards
Learn More About This Simulation
We live in an age of advanced genetic modification where it is possible to generate a mutant organism in a matter of hours. Molecular biologists have been using sophisticated cloning techniques such as uracil-excision (USER) based cloning to create vectors rapidly and efficiently.
Introduction to USER
In this simulation, you will be introduced to the principles of uracil-excision (USER) based cloning. You will learn why USER is superior to other cloning techniques, such as those that involve ligation, and why we are interested in cloning Beta-carotene.
Performing USER
Once you have covered the basics of USER, you will apply your newly-acquired knowledge to clone three genes of the Beta-carotene production pathway into an Escherichia coli strain, in order to start the compound’s production in a cell factory.
By the end of this simulation, you will be able to understand the critical points that must be considered when designing a USER cloning experiment. You will go through the main steps of the USER technique, including designing specific primers, performing the PCR, and choosing specific reagents. Finally, you will also learn how to solve common issues that can arise when using this technique. Will you be able to insert the genes correctly into the desired plasmid?
For Science Programs Providing a Learning Advantage
North Dakota State College of Science
“They did the simulation at home, then completed the in-person lab within 30 minutes, no questions asked, and passed the quiz with flying colors.”
Lecturer in Human Physiology
University of Westminster
"I saw some of the students who clearly didn’t necessarily like sitting there reading a book discover they could turn on Labster and keep up with the rest of the class because it spoke to them.
Modesto City Schools
"Having something that's engaging for the students gives teachers that opportunity to breathe and get excited again. Because they're seeing the kids light up, they're seeing the kids engage with content."
Wenatchee Valley College
"The question always is, ‘Can we demonstrate that the students are meeting course outcomes?’ Check! We can do that.”
San José State University
"We surveyed over 400 students. More than 90% thought Labster was easy to navigate, and that it was fun, but more importantly, most of them felt confident that they could execute the labs in person. And that confidence is a big deal."
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FAQs
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A Labster virtual lab is an interactive, multimedia assignment that students access right from their computers. Many Labster virtual labs prepare students for success in college by introducing foundational knowledge using multimedia visualizations that make it easier to understand complex concepts. Other Labster virtual labs prepare learners for careers in STEM labs by giving them realistic practice on lab techniques and procedures.
Labster’s virtual lab simulations are created by scientists and designed to maximize engagement and interactivity. Unlike watching a video or reading a textbook, Labster virtual labs are interactive. To make progress, students must think critically and solve a real-world problem. We believe that learning by doing makes STEM stick.
Yes, Labster is compatible with all major LMS (Learning Management Systems) including Blackboard, Canvas, D2L, Moodle, and many others. Students can access Labster like any other assignment. If your institution does not choose an LMS integration, students will log into Labster’s Course Manager once they have an account created. Your institution will decide which is the best access method.
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Labster supports a wide range of STEM courses at the high school, college, and university level across fields in biology, chemistry, physics, and health sciences. You can identify topics for your courses by searching our Content Catalog.