USER

USER
Time to complete course: 31 min.
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About the USER Virtual Lab Simulation

In the Uracil-excision (USER) Cloning lab, you will learn about the principles of the Uracil-excision based cloning. You will design specific primers, perform the PCR, and use specific reagents to clone three genes of the Beta-carotene production pathway into an Escherichia coli strain, in order to start the compound’s production in a cell factory. In addition, you will learn how to solve common issues that can arise when using this technique. Will you be able to insert the genes correctly into the desired plasmid?

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Learn about the principles of the Uracil-excision based cloning. Will you be able to insert the genes correctly into the desired plasmid?

Techniques In Lab

  • USER

Learning Objectives

At the end of this simulation, you will be able to…

  • Understand the principles of USER cloning
  • Design primers for USER cloning
  • Understand the molecular mechanism behind the USER cloning
  • Understand the critical points to design a USER cloning experiment

Screenshots of USER Virtual Lab Simulation

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How it works

A million dollar lab in your browser

Perform experiments in virtual lab simulations to achieve core science learning outcomes. 

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Our virtual laboratory simulations are aimed at university, college and high school level, within fields such as biology, biochemistry, genetics, biotechnology, chemistry, physics and more.

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