Time to complete course: 31 min.

About the USER Virtual Lab Simulation

In the Uracil-excision (USER) Cloning lab, you will learn about the principles of the Uracil-excision based cloning. You will design specific primers, perform the PCR, and use specific reagents to clone three genes of the Beta-carotene production pathway into an Escherichia coli strain, in order to start the compound’s production in a cell factory. In addition, you will learn how to solve common issues that can arise when using this technique. Will you be able to insert the genes correctly into the desired plasmid?

Get Started Now

Learn about the principles of the Uracil-excision based cloning. Will you be able to insert the genes correctly into the desired plasmid?

Techniques In Lab

  • USER

Learning Objectives

At the end of this simulation, you will be able to…

  • Understand the principles of USER cloning
  • Design primers for USER cloning
  • Understand the molecular mechanism behind the USER cloning
  • Understand the critical points to design a USER cloning experiment

Screenshots of USER Virtual Lab Simulation

Collaborators

How it works

A million dollar lab in your browser

Perform experiments in virtual lab simulations to achieve core science learning outcomes. 

All our simulations run on laptop and desktop computers, and you can play our simulations without having to install any browser plugins.

See detailed minimum requirements here.

Hundreds of hours of science learning content

Our virtual laboratory simulations are aimed at university, college and high school level, within fields such as biology, biochemistry, genetics, biotechnology, chemistry, physics and more.

With access to our simulations, you will have hundreds of hours of engaging, high-quality learning content available to you.

Discover more Virtual Lab Simulations

We currently have 114 simulations that cover everything from biology and medicine to physics and chemistry.

Learn how you can use Labster in your science course