Uracil based excision cloning (USER): Cloning made simple
About Uracil based excision cloning (USER): Cloning made simple Virtual Lab Simulation
We live in an age of advanced genetic modification where it is possible to generate a mutant organism in a matter of hours. Molecular biologists have been using sophisticated cloning techniques such as uracil-excision (USER) based cloning to create vectors rapidly and efficiently.
Introduction to USER
In this simulation, you will be introduced to the principles of uracil-excision (USER) based cloning. You will learn why USER is superior to other cloning techniques, such as those that involve ligation, and why we are interested in cloning Beta-carotene.
Once you have covered the basics of USER, you will apply your newly-acquired knowledge to clone three genes of the Beta-carotene production pathway into an Escherichia coli strain, in order to start the compound’s production in a cell factory.
By the end of this simulation, you will be able to understand the critical points that must be considered when designing a USER cloning experiment. You will go through the main steps of the USER technique, including designing specific primers, performing the PCR, and choosing specific reagents. Finally, you will also learn how to solve common issues that can arise when using this technique.
Will you be able to insert the genes correctly into the desired plasmid?
Get Started Now
Techniques In Lab
- Uracil-based excision cloning (USER)
At the end of this simulation, you will be able to…
- Understand the principles of USER cloning
- Design primers for USER cloning
- Describe the molecular mechanism behind the USER cloning
- Explain the critical points to design a USER cloning experimens
Screenshots of Uracil based excision cloning (USER): Cloning made simpleVirtual Lab Simulation
How it works
A million dollar lab in your browser
Perform experiments in virtual lab simulations to achieve core science learning outcomes.
All our simulations run on laptop and desktop computers, and you can play our simulations without having to install any browser plugins.
Hundreds of hours of science learning content
Our virtual laboratory simulations are aimed at university, college and high school level, within fields such as biology, biochemistry, genetics, biotechnology, chemistry, physics and more.
With access to our simulations, you will have hundreds of hours of engaging, high-quality learning content available to you.