About This Simulation
In this simulation, you will understand the principles of the Uracil-excision based cloning. You will design specific primers, perform the PCR, and use specific reagents to clone three genes of the Beta-carotene production pathway into an Escherichia coli strain, in order to start the compound’s production in a cell factory. In addition, you will also learn how to solve some common issues that can arise when using this technique. Will you be able to insert the genes correctly into the desired plasmid?