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Bacterial Isolation Virtual Lab

Time to complete course: 35 min.

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Learn about the proper techniques of isolating single colonies from a culture sample, how to use aseptic techniques and how to perform plate streaking.

About the Bacterial Isolation Virtual Lab Simulation

In the Bacterial Isolation simulation, you will investigate the cause of a contamination of poultry meat by a dangerous bacteria strain that is resistant to common antibiotics. After taking samples from the chicken farm, you will work in the virtual laboratory to isolate single colonies of the deadly bacteria among a variety of different species. To do so, you will learn how to work under sterile conditions, and you will be able to practice and perfect your plate streaking technique.

Identifying ampicillin resistant bacteria

The premise of the Bacterial Isolation lab is a report of ampicillin resistant bacteria in poultry meat. You will visit the place of origination, a chicken farm, in hopes of identifying the bacteria strain. The first task is to take a sample, which will contain a variety of bacterial strains, and from that, you must identify which strains are resistant to ampicillin by isolating single colonies

Aseptic technique

In the Bacterial Isolation lab, you will learn how to use aseptic techniques—for example, remembering to turn on the Bunsen burner and sterile their loop in between streaks.

Plate streaking technique

In order to identify the specific bacteria strain, you will have to perform bacterial isolation using the plate streaking technique. There is an unlimited supply of agar plates, giving you the opportunity to practice this technique as many times as you like. Results are given immediately, as opposed to waiting a full 24 hours for incubation, as when performed in reality. You will also streak a special Salmonella Shigella agar. The Salmonella Shigella agar contains a certain medium that only promotes growth of Gram-positive strains. Each of the different strains will exhibit a certain phenotype when grown on the Salmonella Shigella agar. By using this information you can identify the specific strain that is resistant to ampicillin. The sample will then be sent for further analysis to fully confirm the identity.

Will you be able to complete the task with your knowledge on bacterial isolation and successfully isolate the dangerous bacterial strain?

Techniques In Lab

  • Colony screening
  • Sterile technique
  • Plate streaking

 

Learning Objectives

At the end of this simulation, you will be able to…

  • Understand the importance of bacterial growth for the investigation of pathological microorganisms
  • Work under aseptic techniques
  • Understand the concept of a single colony
  • Perform plate-streaking techniques
  • Use selective media for isolation purposes

Screenshots of Bacterial Isolation Virtual Lab Simulation

Collaborators

Mogens Kilstrup

Mogens Kilstrup

Lecturer, Institute for Systems Biology

Techincal University of Denmark

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