Back to simulations

CRISPR-Cas applied to TGF-beta induced EMT | Virtual Lab

Get Pricing
Higher Education
High School
 
CRISPR-Cas applied to TGF-beta induced EMT
Labster is used by 1,000's of amazing schools and universities
Learn more

Heading 1

Heading 2

Heading 3

Heading 4

Heading 5
Heading 6

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur.

Block quote

Ordered list

  1. Item 1
  2. Item 2
  3. Item 3

Unordered list

  • Item A
  • Item B
  • Item C

Text link

Bold text

Emphasis

Superscript

Subscript

About This Simulation

Learn how to detect the hallmarks of EMT using immunofluorescence and knock out the correct gene to revert the process using CRISPR

Learning Objectives

  • Set up a protocol to get answers to a biological question related to the EMT process
  • Acknowledge mistakes in the protocols and understand how to critically overcome them
  • Properly prepare cells for the immunostaining procedure according to the localization of the target protein
  • Select a suitable secondary antibody for an immunofluorescence experiment
  • Analyze immunofluorescence results
  • Describe the basics of CRISPR-Cas technique
  • Design a guide RNA construct for knock-out strategies
  • Evaluate CRISPR-Cas9 results

About This Simulation

Level:
Higher Education
High School
Length:
49
Min
Accessibility Mode:
Available
Languages:
English

Lab Techniques

  • CRISPR-Cas9
  • Immunofluorescence
No lab techniques are listed for this simulation.

Related Standards

University:
NGSS:
  • No direct alignment
AP:
  • No direct alignment
LB:
  • No direct alignment
No lab techniques are listed for this simulation.

Learn More About This Simulation

CRISPR may sound like a brand of cereals, but it certainly isn’t! In this simulation, you will learn to identify the molecular hallmarks of the Epithelial-to-Mesenchymal Transition (EMT) molecular process in breast cancer cells. You will induce EMT by adding TGF-beta to the cells, and, after identifying the hallmarks with immunofluorescence labeling, you will use the cutting-edge CRISPR-Cas9 technology to try to revert this process, which is one of the main causes of cancer malignancy.

Set up your own immunofluorescence protocol

In your first mission, you will be able to perform an immunostaining protocol by making decisions on the key steps of the process. Each decision can get you closer to the correct outcome, so think carefully and choose wisely!

Use CRISPR to knock out a specific gene

Once you have learned what the molecular hallmarks of EMT are, you will be able to dive into a eukaryotic cell to learn how the CRISPR-Cas9 technique works at a molecular level. Then, you will learn to design one of the CRISPR-Cas9 components by solving a fun puzzle. Last, you will select the target gene to knock out and your virtual lab assistant, Dr. One, will help you with the last part of the immunostaining process so you can directly evaluate your results.

Repeat your experiment as many times as you want

At the end of each mission, you will be able to repeat the experiment if the outcome was not the desired one, giving you the possibility of learning from your mistakes. Luckily, Dr. One will be there to help you with some of the tasks. In the end, you will be able to complete an experiment that takes weeks in just less than one hour!

Will you be able to set the right protocols in place, solving any issues that arise, to understand the molecular changes linked to the TGF-beta induced EMT and evaluate the possibility of using CRISPR-Cas9 as a possible therapeutic approach in breast cancer?

Experience Labster for Yourself

Boost Learning with Fun

75% of students show high engagement and improved grades with Labster

Discover Simulations That Match Your Syllabus

Easily bolster your learning objectives with relevant, interactive content

Place Students in the Shoes of Real Scientists

Practice a lab procedure or visualize theory through narrative-driven scenarios

Try Now
a group of people standing around a laptop computer

For Science Programs Providing a Learning Advantage

Professor Margaret Brady was able to enhance student learning with A&P virtual labs.
Margaret Brady
Associate Professor
North Dakota State College of Science

“They did the simulation at home, then completed the in-person lab within 30 minutes, no questions asked, and passed the quiz with flying colors.”

Lewis Mattin
PhD
Lecturer in Human Physiology
University of Westminster

"I saw some of the students who clearly didn’t necessarily like sitting there reading a book discover they could turn on Labster and keep up with the rest of the class because it spoke to them.

Melody McGill
Curriculum Coordinator
Modesto City Schools

"Having something that's engaging for the students gives teachers that opportunity to breathe and get excited again. Because they're seeing the kids light up, they're seeing the kids engage with content."

user
Kyle Hammon
Adjunct Instructor
Wenatchee Valley College

"The question always is, ‘Can we demonstrate that the students are meeting course outcomes?’ Check! We can do that.”

Dr. Melody Esfandiari
Chemistry Lecturer
San José State University

"We surveyed over 400 students. More than 90% thought Labster was easy to navigate, and that it was fun, but more importantly, most of them felt confident that they could execute the labs in person. And that confidence is a big deal."

Mr stuart
Dr. Stuart Goodall
Lecturer
Northumbria University

“The Labster simulations get students to do things, and they're not just sitting there consuming a webinar where their mind can drift. They become an active participant in that learning experience.”

FAQs

Find answers to frequently asked questions.

Heading 1

Heading 2

Heading 3

Heading 4

Heading 5
Heading 6

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur.

Block quote

Ordered list

  1. Item 1
  2. Item 2
  3. Item 3

Unordered list

  • Item A
  • Item B
  • Item C

Text link

Bold text

Emphasis

Superscript

Subscript

How do students access Labster?

Labster is hosted online, which means that students only have to login from their internet browsers once an account is created.

How is Labster purchased?

Labster is only available for purchase by faculty and administration at academic institutions. To procure Labster, simply reach out to us on our website. Schedule a demo, book a meeting to discuss pricing, start a free trial, or simply fill out our contact form.

How is Labster different from other learning solutions?

Labster simulations are created by real scientists and designed with unparalleled interactivity. Unlike point and click competitors, Labster simulations immerse students and encourage mastery through active learning.

What types of courses does Labster support?

Labster supports a wide range of courses at the high school and university level across fields in biology, chemistry and physics. Some simulations mimic lab procedures with high fidelity to train foundational skills, while others are meant to bring theory to life through interactive scenarios.