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RNA Extraction: Sample and purify mRNA from pigs Virtual Lab

Learn how to extract RNA from pig fat tissue samples and how to purify messenger RNA using magnetic beads.

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RNA Extraction: Sample and purify mRNA from pigs

About this Simulation

How do you know which genes in your DNA are active in your cells? In this simulation, you will learn how to extract messenger RNA molecules, the direct product of your genes.

Extracting mRNA from pigs

You will first retrieve samples of fat tissue from pigs at a farm. Back in the lab, you will then break the cells to extract the total RNA. FInally, you will purify the messenger RNA from the rest of the RNA in your sample.

mRNA extraction and purification

First, the students will learn how to lyse cells, extract RNA using a chloroform solution and purify mRNA with magnetic beads.

Students are immediately provided with qualitative data (spectrophotometer, electrophoresis) to check the quality of their samples.

Learning Objectives

At the end of this simulation, you will be able to:

  • Understand how to extract the total RNA from a cell

  • Separate mRNA molecules specifically from the rest of the RNA

Techniques in Lab

At the end of this simulation, you will be able to:

  • Chloroform Extraction
  • Magnetic Beads Purification

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