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About This Simulation
Learn how to extract RNA from pig fat tissue samples and how to purify messenger RNA using magnetic beads.
Learning Objectives
- Understand how to extract the total RNA from a cell
- Separate mRNA molecules specifically from the rest of the RNA
About This Simulation
Lab Techniques
- Chloroform Extraction
- Magnetic Beads Purification
Related Standards
- No direct alignment
- No direct alignment
- No direct alignment
Learn More About This Simulation
How do you know which genes in your DNA are active in your cells? In this simulation, you will learn how to extract messenger RNA molecules, the direct product of your genes.
Extracting mRNA from pigs
You will first retrieve samples of fat tissue from pigs at a farm. Back in the lab, you will then break the cells to extract the total RNA. FInally, you will purify the messenger RNA from the rest of the RNA in your sample.
mRNA extraction and purification
First, the students will learn how to lyse cells, extract RNA using a chloroform solution and purify mRNA with magnetic beads.
Students are immediately provided with qualitative data (spectrophotometer, electrophoresis) to check the quality of their samples.
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Investigate each step of SDS-PAGE from gel selection and sample preparation to chamber assembly and what really happens when the current turns on, to separate proteins solely by molecular weight, bringing us one step closer to identifying the protein.
RNA Extraction: Sample and purify mRNA from pigs
Learn how to extract RNA from pig fat tissue samples and how to purify messenger RNA using magnetic beads.
For Science Programs Providing a Learning Advantage
North Dakota State College of Science
“They did the simulation at home, then completed the in-person lab within 30 minutes, no questions asked, and passed the quiz with flying colors.”
Lecturer in Human Physiology
University of Westminster
"I saw some of the students who clearly didn’t necessarily like sitting there reading a book discover they could turn on Labster and keep up with the rest of the class because it spoke to them.
Modesto City Schools
"Having something that's engaging for the students gives teachers that opportunity to breathe and get excited again. Because they're seeing the kids light up, they're seeing the kids engage with content."
Wenatchee Valley College
"The question always is, ‘Can we demonstrate that the students are meeting course outcomes?’ Check! We can do that.”
San José State University
"We surveyed over 400 students. More than 90% thought Labster was easy to navigate, and that it was fun, but more importantly, most of them felt confident that they could execute the labs in person. And that confidence is a big deal."
FAQs
Find answers to frequently asked questions.
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A Labster virtual lab is an interactive, multimedia assignment that students access right from their computers. Many Labster virtual labs prepare students for success in college by introducing foundational knowledge using multimedia visualizations that make it easier to understand complex concepts. Other Labster virtual labs prepare learners for careers in STEM labs by giving them realistic practice on lab techniques and procedures.
Labster’s virtual lab simulations are created by scientists and designed to maximize engagement and interactivity. Unlike watching a video or reading a textbook, Labster virtual labs are interactive. To make progress, students must think critically and solve a real-world problem. We believe that learning by doing makes STEM stick.
Yes, Labster is compatible with all major LMS (Learning Management Systems) including Blackboard, Canvas, D2L, Moodle, and many others. Students can access Labster like any other assignment. If your institution does not choose an LMS integration, students will log into Labster’s Course Manager once they have an account created. Your institution will decide which is the best access method.
Labster is available for purchase by instructors, faculty, and administrators at education institutions. Purchasing our starter package, Labster Explorer, can be done using a credit card if you are located in the USA, Canada, or Mexico. If you are outside of North America or are choosing a higher plan, please speak with a Labster sales representative. Compare plans.
Labster supports a wide range of STEM courses at the high school, college, and university level across fields in biology, chemistry, physics, and health sciences. You can identify topics for your courses by searching our Content Catalog.