Select Page
Enzyme Kinetics
Investigate “Asian Glow” syndrome by studying Alcohol Dehydrogenase kinetics. Learn how to use a spectrophotometer, perform enzyme kinetics experiment, analyze data and understand different inhibition mechanisms.

About This Simulation

In the Enzyme Kinetics Lab, students learn how substrates are converted into products by catalysis. Additionally, students learn about the kinetics of enzyme involving the Michaelis-Menten equation and various rate constants. Content regarding DNA mutation and hyperactivity are also covered. In this lab, students run experiments using the enzyme Alcohol Dehydrogenase on a wild and mutant type to learn about Asian Glow syndrome.

Screenshots

Collaborators

Prof. Karen Skriver

Department of biology

University of Copenhagen

Dr. Gert Dandanell

Department of biology

University of Copenhagen

Dr. Lasse Kristoffer Bak

School of Pharmaceutical Sciences

University of Copenhagen

Dr. Michael May

Department of Science Education

University of Copenhagen

Dr. Susanne Jacobsen

Institute for System Biology

Technical University of Denmark

Dr. Maher Abou Hachem

Institute for System Biology

Technical University of Denmark

Learning Objectives

  • Learning the experimental design of enzyme kinetics
  • Understanding the Michaelis-Menten model of enzyme kinetics
  • Analyzing spectrophotometer data and calculate Km and Vmax
  • Understanding that kinetics of an enzyme can be modified by genetic mutations
  • Understanding inhibition kinetics by using several types of inhibitors

Techniques

  • Spectrophotometry
  • Data analysis of enzyme kinetics measurements

Collaborator

Collaborator

List of all our learning simulations

View All Simulations