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FACS
In the Fluorescence-automated Cell Sorting (FACS) Simulation, you will learn the basics of flow cytometry and how to use a flow cytometer with fluorescence detection.

About This Simulation

In the Fluorescence-automated Cell Sorting (FACS) Simulation, you will learn the basics of flow cytometry and how to use a flow cytometer with fluorescence detection. Your goal is to sort a pool of Escherichia coli expressing Green Fluorescent Protein (GFP) at different levels after it has been previously engineered. You will set up the equipment, learn how the cytometer sorts the cells, interpret your results, and clean and shut down the equipment. Will you be able to perform all the steps correctly and sort the cells depending on their GFP expression level?

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Learning Objectives

  • Understanding the basics of flow cytometry technique
  • Understanding the importance of each system that the technique relies on (fluidics, optics, and electronics)
  • Learning to use a cell sorter (Start up, sorting, and shut down of the equipment)
  • Understanding how the equipment performs the measurements
  • Learning to interpret the results and understanding the applications of the technique

Techniques

  • Flow cytometry

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