FACS

Fluorescence-activated cell sorting (FACS) is a specialized type of flow cytometry. It provides a method for sorting a heterogeneous mixture of cells, one at a time, based upon the specific light scattering and fluorescent characteristics of each cell. It provides fast, objective, and quantitative recording of fluorescent signals from individual cells, as well as physical separation of cells of particular interest. Using the light scattering, FACS can detect cell size and complexity, while the fluorescence characteristics allow the researchers to analyze different biological functions or study specific cell components. As a flow cytometry technique, FACS is also based on three main systems: fluidics, optics, and electronics. Their combined work allows the process of cell sorting.

In the Fluorescence-automated Cell Sorting (FACS) Simulation, you will learn the basics of flow cytometry and how to use a flow cytometer with fluorescence detection. Your goal is to sort a pool of Escherichia coli expressing Green Fluorescent Protein (GFP) at different levels after it has been previously engineered. You will set up the equipment, learn how the cytometer sorts the cells, interpret your results, and clean and shut down the equipment. Will you be able to perform all the steps correctly and sort the cells depending on their GFP expression level?