Select Page
Perform aerobic and anaerobic fermentation to produce second-generation bioethanol. Experiment how different parameters affect the rate of fermentation.

About This Simulation

Fermentation technology can be used to produce valuable biological compounds in large quantities. For instance, most antibiotics are produced using fermentation technology. Desired compounds used in most antibiotics are produced in large tanks, in which fungi grows. To ensure quality production, fungi are grown under specific conditions to promote production of the desired compound. Fermentation can be used to produce pharmaceuticals, bioethanol, biochemicals, enzymes and many other products. Ensuring that cells have optimal conditions for production is not a trivial task. Many theories, mathematical models, and advanced laboratory and production equipment have been developed to allow and increase production. In this lab, students will setup fermentation of a second-generation bioethanol production plant that converts waste into bioethanol. Students make a pilot scale experiment and develop a process for efficient bioethanol production. By the end of the lab, students will have a thorough understanding of Fermentation Technology and its application.



Dr. Jette Thykær

Department of Systems Biology

Technical University of Denmark

Learning Objectives

  • Understanding cell growth, goals of fermentation and application to the real-world
  • Understanding the function and various parts of the bioreactor and auxiliary equipment
  • Understanding microbial growth kinetics with examples of batch and chemostat fermentations
  • Understanding how parameters such as pH, temperature, aeration, and agitation affects fermentation
  • Performing virtual fermentations to identify optimal process conditions


  • Yeast inoculation
  • Sterile microbiology technique
  • Graphing
  • Microbe identification
  • Fermentation


List of all our learning simulations

View All Simulations