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Genetically Engineered Machine

In this simulation you will join an iGEM team to design a biosensor that can sense biofilm forming bacteria.

About This Simulation

The International Genetically Engineered Machine (iGEM) competition is a worldwide synthetic biology competition that was initially aimed at undergraduate university students, but has since expanded to include divisions for high school students, entrepreneurs, and community laboratories, as well as ‘overgraduates’.

In this simulation you will join an iGEM team to design a biosensor that can sense biofilm forming bacteria. You will combine two BioBrick parts and transform bacteria with the gene construct. Will you be able to create a novel synthetic organism that can be used as a biosensor?

Screenshots

Collaborators

Kirsten Jensen, PhD

Kirsten Jensen, PhD

Project Manager

SynbiCITE, Imperial College London

Ke Yan Wen, PhD student

Ke Yan Wen, PhD student

Centre for Synthetic Biology and Innovation

Imperial College London

Learning Objectives

  • Planning your iGEM project together with your team
  • Learning how BioBricks can be assembled using restriction enzymes
  • Learning how to transform a gene construct into bacteria cells
  • Experimenting with different restriction enzymes and trying to clone a new device

Techniques In This Simulation

  • 3A-Assembly
  • Heat shock transformation
  • Antibiotics selection
  • Restriction digest
  • Ligation

Collaborator

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