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Genetically Engineered Machine
In this simulation you will join an iGEM team to design a biosensor that can sense biofilm forming bacteria.
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About This Simulation

In this simulation you will join an iGEM team to design a biosensor that can sense biofilm forming bacteria. You will combine two BioBrick parts and transform bacteria with the gene construct. Will you be able to create a novel synthetic organism that can be used as a biosensor?

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Collaborators

Kirsten Jensen, PhD

Project Manager

SynbiCITE, Imperial College London

Ke Yan Wen, PhD student

Centre for Synthetic Biology and Innovation

Imperial College London

Learning Objectives

  • Planning your iGEM project together with your team
  • Learning how BioBricks can be assembled using restriction enzymes
  • Learning how to transform a gene construct into bacteria cells
  • Experimenting with different restriction enzymes and trying to clone a new device

Techniques

  • 3A-Assembly
  • Heat shock transformation
  • Antibiotics selection
  • Restriction digest
  • Ligation

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