HPLC

Time to complete course: 52 min.
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About HPLC Virtual Lab Simulation

Explore the HPLC machine by taking a look inside it. Find the different components including the sample injection station, column, pumps and UV detector, and learn all about the principles of liquid chromatography, specifically HPLC.

Understanding High Performance Liquid Chromatography

In the HPLC simulation, your first task is to select different mobile phases and different columns to run your experiment. You will then separate the components of a drug and measure concentrations. Next, compare the separation profile using different mobile phases and columns. During the experiment, you will be able to visualize the lipophilic interaction between analyte and both the stationary and mobile phase by watching a 3D animation. Quiz questions throughout the lab check will check your understanding of the HPLC technique.

Drug stability analysis

After completing the first part of the HPLC lab, you will learn how to analyze drugs that have been stored in different conditions and measure and compare the active ingredient’s concentration.

About the HPLC lab

The HPLC Lab is constructed with specific detail and information that can be beneficial for anyone pursuing pharmaceutical studies. Additionally, it can be applied to any area of study that uses chromatography techniques such as biology and biochemistry. Chromatography is an important technique used by pharmacists, and drug stability is an important issue that a pharmacist must be aware of. This lab combines the technique and application.

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Learn how different factors such as heat and humidity can alter drug stability. Identify the components of the HPLC machine and use it to separate and measure the different compounds of a medicine.

Techniques in Lab

  • HPLC

Learning Objectives

At the end of this simulation, you will be able to…

  • Understand the different compartments of an HPLC machine and functions
  • Understand the principles of HPLC separation
  • Understand the lipophilic interaction between the analyte and the mobile and stationary phase
  • Understand the different changes in parameters (such as the column, mobile phase, temperature etc.) and its effects on analyte separation and concentration measurements

Simulation Features

Length – 52 minutes
Languages – English

Examples of Related Standards

University

Life Science related 2nd year onwards

NGSS

HS-PS1-3, HS-PS2-6

IB

B.9 Biological Pigments

AP

3.9 Separation of Solutions and Mixtures Chromatography

Screenshots of HPLC Virtual Lab Simulation

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