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HPLC

Learn about how factors, such as heat and humidity, can alter drug stability. Identify the components of the HPLC machine and use it to separate – and measure the different compounds of a medicine.

About This Simulation

In the first part of the exercise, students explore the HPLC machine. They can open various parts of the machine and learn about the different components including the sample injection station, column, pumps and UV detector. By understanding how the machine works, students will be able to understand the principles of liquid chromatography, specifically HPLC.

Students will then select different mobile phases and different columns to run their experiment. They will separate the components of a drug and measure concentrations. They will then compare the separation profile using different mobile phases and columns. During the experiment students will be able to visualize the lipophilic interaction between analyte and both the stationary and mobile phase by watching a 3D animation. Quiz questions throughout the lab check the student’s understanding of the HPLC technique.

After completing the first part of the exercise, students continue to analyze drugs that have been stored in different conditions. Students will measure and compare the active ingredient’s concentration.

The HPLC Lab is constructed with specific detail and information that can be beneficial for students pursuing pharmaceutical studies. Additionally, it can be applied to any area of study that uses chromatography techniques such as biology and biochemistry. Chromatography is an important technique used by pharmacists, and drug stability is an important issue that a pharmacist must be aware of. This lab combines the technique and application.

Understanding High Performance Liquid Chromatography

In the first part of the exercise, students explore the HPLC machine. They can open various parts of the machine and learn about the different components including the sample injection station, column, pumps and UV detector. By understanding how the machine works, students will be able to understand the principles of liquid chromatography, specifically HPLC.

Students will then select different mobile phases and different columns to run their experiment. They will separate the components of a drug and measure concentrations. They will then compare the separation profile using different mobile phases and columns. During the experiment students will be able to visualize the lipophilic interaction between analyte and both the stationary and mobile phase by watching a 3D animation. Quiz questions throughout the lab check the student’s understanding of the HPLC technique.

Drug stability analysis

After completing the first part of the exercise, students continue to analyze drugs that have been stored in different conditions. Students will measure and compare the active ingredient’s concentration.

The HPLC lab is constructed with specific detail and information that can be beneficial for students pursuing pharmaceutical studies. Additionally, it can be applied to any area of study that uses chromatography techniques such as biology and biochemistry. Chromatography is an important technique used by pharmacists, and drug stability is an important issue that a pharmacist must be aware of. This lab combines the technique and application.

Screenshots

Collaborators

Learning Objectives

  • Learning the different compartments of an HPLC machine and functions
  • Learning the principles of HPLC separation
  • Understanding the lipophilic interaction between the analyte and the mobile and stationary phase
  • Understanding the different changes in parameters (such as the column, mobile phase, temperature etc.) and its effects on analyte separation and concentration measurements

Techniques In This Simulation

  • HPLC

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