Intestinal Glucose Transport: Study a mouse intestine model to diagnose an infant

Intestinal Glucose Transport
Time to complete course: 30 min.

About the Intestinal Glucose Transport Virtual Lab Simulation

In this simulation, you will help treat an infant who is experiencing diarrhea, and as a consequence, is not gaining weight. She also has elevated blood sodium concentration and glucose in her feces and urine. Dr. Shaw, your supervisor, suspects that her diarrhea is caused by a lack of ability to absorb glucose. In this simulation, you will use a mouse model to study glucose transport and see if data from animal studies can be used to diagnose human diseases.

Make an everted sac

You will use a piece of mouse intestine as your model to study glucose transport between the inner (mucosal) and outer (serosal) sides. To make sure no impurities interfere with your results, you will need to turn it inside out, which you will do via an interactive animation. At the end of the everting procedure, the intestine looks like a sausage!

Study glucose transport by measuring its concentration

Once you have your everted intestinal sac, you will expose both sides to glucose solutions of different concentrations, a sodium-potassium ATPase blocker and a sodium-free saline solution. Then, after measuring the glucose levels on both sides following the treatments, you will analyze your data and be able to understand how active glucose transport in the intestinal epithelium works. A step-by-step interactive summary diagram will help you visualize what happens to all the molecules and transporters involved.

Help diagnose the infant

Once you know how intestinal active glucose transport works and what molecules are involved, you will call Dr. Shaw so that she can confirm your diagnosis and try to treat her little patient.

Will your findings be able to help make the infant well again?

Get Started Now

Turn a mouse intestine inside out and use it to study glucose transport in order to figure out what is wrong with an infant.

Techniques In Lab

  • Make an everted intestinal sac
  • Use a mouse intestinal everted sac to study epithelial glucose transport between mucosal and serosal sides
  • Calibration curve
  • Glucose assay (oxidase, peroxidase, o-dianisidine)

Learning Objectives

At the end of this simulation, you will be able to…

  • Understand the epithelial model for how glucose is transported across the mammalian small intestine
  • Perform a glucose assay method
  • Use an animal model to study the transport of materials across the intestine
  • Describe the effect of blocker ouabain on glucose transport by the small intestine
  • Explain the effect of manipulations of mucosal concentrations of glucose and sodium on glucose intestinal transport
  • Interpret physiological data and apply to clinical cases

Screenshots of Intestinal Glucose Transport Virtual Lab Simulation


Dr. Jon Harrison

School of Life Sciences
Arizona State University

How it works

A million dollar lab in your browser

Perform experiments in virtual lab simulations to achieve core science learning outcomes. 

All our simulations run on laptop and desktop computers, and you can play our simulations without having to install any browser plugins.

See detailed minimum requirements here.

Hundreds of hours of science learning content

Our virtual laboratory simulations are aimed at university, college and high school level, within fields such as biology, biochemistry, genetics, biotechnology, chemistry, physics and more.

With access to our simulations, you will have hundreds of hours of engaging, high-quality learning content available to you.

Discover more Virtual Lab Simulations

We currently have 133 simulations that cover everything from biology and medicine to physics and chemistry.

Learn how you can use Labster in your science course

Are you looking to bring a course online in response to COVID-19?