Intestinal Glucose Transport: Study a mouse intestine model to diagnose an infant
About Intestinal Glucose Transport: Study a mouse intestine model to diagnose an infant Virtual Lab Simulation
In this simulation, you will help treat an infant who is experiencing diarrhea, and as a consequence, is not gaining weight. She also has elevated blood sodium concentration and glucose in her feces and urine. Dr. Shaw, your supervisor, suspects that her diarrhea is caused by a lack of ability to absorb glucose. In this simulation, you will use a mouse model to study glucose transport and see if data from animal studies can be used to diagnose human diseases.
Make an everted sac
You will use a piece of mouse intestine as your model to study glucose transport between the inner (mucosal) and outer (serosal) sides. To make sure no impurities interfere with your results, you will need to turn it inside out, which you will do via an interactive animation. At the end of the everting procedure, the intestine looks like a sausage!
Study glucose transport by measuring its concentration
Once you have your everted intestinal sac, you will expose both sides to glucose solutions of different concentrations, a sodium-potassium ATPase blocker and a sodium-free saline solution. Then, after measuring the glucose levels on both sides following the treatments, you will analyze your data and be able to understand how active glucose transport in the intestinal epithelium works. A step-by-step interactive summary diagram will help you visualize what happens to all the molecules and transporters involved.
Help diagnose the infant
Once you know how intestinal active glucose transport works and what molecules are involved, you will call Dr. Shaw so that she can confirm your diagnosis and try to treat her little patient.
Will your findings be able to help make the infant well again?
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Techniques In Lab
- Make an everted intestinal sac
- Use a mouse intestinal everted sac to study epithelial glucose transport between mucosal and serosal sides
- Calibration curve
- Glucose assay (oxidase, peroxidase, o-dianisidine)
At the end of this simulation, you will be able to…
- Understand the epithelial model for how glucose is transported across the mammalian small intestine
- Perform a glucose assay method
- Use an animal model to study the transport of materials across the intestine
- Describe the effect of blocker ouabain on glucose transport by the small intestine
- Explain the effect of manipulations of mucosal concentrations of glucose and sodium on glucose intestinal transport
- Interpret physiological data and apply to clinical cases
Screenshots of Intestinal Glucose Transport: Study a mouse intestine model to diagnose an infantVirtual Lab Simulation
Dr. Jon HarrisonSchool of Life Sciences Arizona State University
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