Measure the protein content in a food sample with the Kjeldahl method, and investigate whether the apparent result has been tampered with by using LC-MS/MS.
University / College
In the Kjeldahl Method simulation, you will learn how to use the Kjeldahl method to determine the protein content of a food sample, and how to use LC-MS/MS to investigate if the sample has been adulterated to make the protein content seem higher than it is.
Analyze a milk powder sample
Your lab has received batch samples of milk powder from 3 different producers. Your mission is to analyze a sample from one of the batches using the Kjeldahl method, in order to determine the exact protein content, and to make sure this is consistent with the reported value.
Tracing the steps of the Kjeldahl method
An animation will introduce you to each step of the analysis, and show what goes on at the molecular level.You will then perform the acid digestion, steam distillation and finally a colorimetric titration, before being challenged with the calculations needed to transfer the lab result to a protein content in the sample.
Is everything what it appears to be?
How can you be certain that your findings are correct? There might be ways to fool the Kjeldahl method! In the second half of the simulation, you will look into how this can happen, and explore the powerful technique LC-MS/MS, which can be used to detect if a known adulterant has been added to any of the received milk powder batches.
Will you be able to safely release the milk powder batches for consumers around the world?
Length:
45
mins
Accessibility mode:
Available
Languages:
English (United States)
Course Packages:
At the end of this simulation, you will be able to:
Describe the steps involved in the Kjeldahl method
Analyze the nitrogen content of a food sample
Calculate the protein content of a sample from the determined nitrogen content
Explain why the Kjeldahl method cannot be used to identify milk powder that was cut with melamine
Apply the principles of spiking and recovery in analytical chemistry
Interpret basic LC-MS/MS data
Describe the fundamentals of an LC-MS/MS analysis
Argue whether food fraud has been conducted based on available data
At the end of this simulation, you will be able to:
University
NGSS
IB
AP
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