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Mass Spectrometry 1: Sample Preparation

You will assist in a biopsy on a cancer patient and learn how to prepare the sample for mass spectrometry by extracting its phosphopeptides. Will you be able to identify the cancer stage of the patient by looking at the phosphorylation pattern?

About This Simulation

The MS Sample Preparation simulation is the first simulation in the Mass Spectrometry series. Here, you will assist in a biopsy on a cancer patient and learn how to prepare the sample for mass spectrometry by extracting its phosphopeptides. Will you be able to identify the cancer stage of the patient by looking at the phosphorylation pattern?

Screenshots

Collaborators

Dr. James Longden

Dr. James Longden

Biotech Research and Innovation Centre

University of Copenhagen

Learning Objectives

  • Understanding the correlation between phosphorylation and cancer
  • Learning how to prepare a biopsy sample for mass spectromerty

Techniques

  • Cell lysis
  • Protein purification
  • Enzymatic digestion
  • Phosphopeptide enrichment

Collaborator

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