About Cancer Sample Preparation for Mass Spectrometry Virtual Lab Simulation
Take action! Cancer is on the rise, and you can help with diagnosis and treatment. In the Cancer Sample Preparation for Mass Spectrometry simulation, you will assist doctors by determining the cancer stage of a patient diagnosed with colon cancer. This is the first simulation in the Mass Spectrometry series.
Prepare the biopsy
Join doctors in the hospital and enter an immersive animation to see how we perform a colorectal cancer biopsy. Once back in the lab, your mission will be to extract proteins from the tumor sample. Find the equipment you need in the lab to lyse the tumor cells, denature the proteins, and perform phosphopeptide enrichment, in order to prepare the sample for mass spectrometry.
Explore the molecules
Use the holotable to observe detailed 3D models of the molecules involved in phosphorylation. Get an understanding of the molecular principles underlying the biopsy preparation and how the phosphorylation pattern relates to cancer.
Interpret your findings
In the end, you will analyze the results from the mass spectrometry on a graph. Will you be able to help guide the patient’s treatment by identifying their cancer stage?
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Techniques In Lab
- Cell lysis
- Protein purification
- Enzymatic digestion
- Phosphopeptide enrichment
Learning Objectives
At the end of the simulation, you will be able to…
- Understand the correlation between phosphorylation and cancer
- Prepare a biopsy sample for mass spectrometry
Collaborators
Dr. James Longden
Biotech Research and Innovation Centre University of CopenhagenHow it works
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