Back to simulations

Molecular Cloning | Virtual Lab

Get Pricing
Higher Education
 
Molecular Cloning
Labster is used by 1,000's of amazing schools and universities
Learn more

Heading 1

Heading 2

Heading 3

Heading 4

Heading 5
Heading 6

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur.

Block quote

Ordered list

  1. Item 1
  2. Item 2
  3. Item 3

Unordered list

  • Item A
  • Item B
  • Item C

Text link

Bold text

Emphasis

Superscript

Subscript

About This Simulation

Dive into recombinant DNA technology with cell division, transcription and translation. Includes concepts in restriction enzymes, cloning and reporter genes.

Learning Objectives

  • Understand molecular cloning techniques: DNA extraction and preparation, ligation, transformation, plate streaking and antibiotic selection
  • Understand inducible gene expression regulation
  • Understand the use of GFP as a reporter gene
  • Understand DNA damage and DNA repair system

About This Simulation

Level:
Higher Education
Length:
57
Min
Accessibility Mode:
Available
Languages:
English
Italian
Spanish
French
German

Lab Techniques

  • Transformation
  • Colony screening
  • DNA extraction
  • Cloning
No lab techniques are listed for this simulation.

Related Standards

University:
  • Early Stage Bachelors Level
  • EHEA First Cycle
  • FHEQ 6
  • Intermediate Stage Bachelors Level
  • Late Stage Bachelors Level
  • US College Year 1
  • US College Year 2
  • US College Year 3
  • US College Year 4
NGSS:
  • No direct alignment
AP:
  • No direct alignment
LB:
  • No direct alignment
No lab techniques are listed for this simulation.

Learn More About This Simulation

Molecular cloning is one of the techniques that has laid the foundation for modern biotechnology. The technique was first used in the 1980′s and allowed the insertion of an insulin gene derived from humans to be inserted into yeast and coli bacteria. This allowed the microbes to produce insulin, which is the primary medication in diabetes treatment. Since then, molecular cloning and genetic engineering has become one of the most fundamental techniques ranging from pharmaceutical production, bioethanol production along with medical to basic research. In the Molecular Cloning lab, you will learn how to assemble an expression vector containing RAD52 and GFP. The aim is to control the expression level of RAD52 with Doxycyline and to monitor the expression level by observing the GFP signal.

Vector assembly

In the first part of the Molecular Cloning lab, you will learn how to extract DNA from yeast cells and restrict enzyme isolation in DNA from another vector. First, you will prepare the extracted DNA and measure the concentration, and then, you will assemble a vector containing a gene of interest (RAD52) and GFP using the correct ligase, buffer and temperature of incubation.

Transformation

The assembled vector will be transformed into yeast cells using electroporation. RAD52 gene expression is regulated by a gene regulator. When Doxycyline is added to the media, RAD52 gene will be silenced. GFP is used as a reporter gene to RAD52, cells with active RAD52 will also express GFP and cells with silenced RAD52 will not express GFP. The GFP signal is monitored by exposing the cells to blue light.

DNA damage and repair system

RAD52 is hypothesized to be an important player in DNA repair. You will perform an experiment comparing the result of induced DNA damage through UV radiation in cells expressing RAD52 and cells with silenced RAD52. If RAD52 is important in performing DNA repair, cells with silenced RAD52 will not survive the UV radiation treatment. All in all, the Molecular Cloning lab will give you an overview of the molecular cloning techniques and the reporter gene, and you will learn all about DNA damage and DNA repair system.

Experience Labster for Yourself

Boost Learning with Fun

75% of students show high engagement and improved grades with Labster

Discover Simulations That Match Your Syllabus

Easily bolster your learning objectives with relevant, interactive content

Place Students in the Shoes of Real Scientists

Practice a lab procedure or visualize theory through narrative-driven scenarios

Try Now
a group of people standing around a laptop computer

For Science Programs Providing a Learning Advantage

Professor Margaret Brady was able to enhance student learning with A&P virtual labs.
Margaret Brady
Associate Professor
North Dakota State College of Science

“They did the simulation at home, then completed the in-person lab within 30 minutes, no questions asked, and passed the quiz with flying colors.”

Lewis Mattin
PhD
Lecturer in Human Physiology
University of Westminster

"I saw some of the students who clearly didn’t necessarily like sitting there reading a book discover they could turn on Labster and keep up with the rest of the class because it spoke to them.

Melody McGill
Curriculum Coordinator
Modesto City Schools

"Having something that's engaging for the students gives teachers that opportunity to breathe and get excited again. Because they're seeing the kids light up, they're seeing the kids engage with content."

user
Kyle Hammon
Adjunct Instructor
Wenatchee Valley College

"The question always is, ‘Can we demonstrate that the students are meeting course outcomes?’ Check! We can do that.”

Dr. Melody Esfandiari
Chemistry Lecturer
San José State University

"We surveyed over 400 students. More than 90% thought Labster was easy to navigate, and that it was fun, but more importantly, most of them felt confident that they could execute the labs in person. And that confidence is a big deal."

Mr stuart
Dr. Stuart Goodall
Lecturer
Northumbria University

“The Labster simulations get students to do things, and they're not just sitting there consuming a webinar where their mind can drift. They become an active participant in that learning experience.”

FAQs

Find answers to frequently asked questions.

Heading 1

Heading 2

Heading 3

Heading 4

Heading 5
Heading 6

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur.

Block quote

Ordered list

  1. Item 1
  2. Item 2
  3. Item 3

Unordered list

  • Item A
  • Item B
  • Item C

Text link

Bold text

Emphasis

Superscript

Subscript

How do students access Labster?

Labster is hosted online, which means that students only have to login from their internet browsers once an account is created.

How is Labster purchased?

Labster is only available for purchase by faculty and administration at academic institutions. To procure Labster, simply reach out to us on our website. Schedule a demo, book a meeting to discuss pricing, start a free trial, or simply fill out our contact form.

How is Labster different from other learning solutions?

Labster simulations are created by real scientists and designed with unparalleled interactivity. Unlike point and click competitors, Labster simulations immerse students and encourage mastery through active learning.

What types of courses does Labster support?

Labster supports a wide range of courses at the high school and university level across fields in biology, chemistry and physics. Some simulations mimic lab procedures with high fidelity to train foundational skills, while others are meant to bring theory to life through interactive scenarios.