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Molecular Cloning
Dive into recombinant DNA technology with cell division, transcription and translation. Includes concepts in restriction enzymes, cloning and reporter genes.

About This Simulation

Molecular cloning is one of the techniques that has laid the foundation for modern biotechnology. The technique was first used in the 1980′s and allowed the insertion of an insulin gene derived from humans to be inserted into yeast and coli bacteria. This allowed the microbes to produce insulin, which is the primary medication in diabetes treatment. Since then, molecular cloning and genetic engineering has become one of the most fundamental techniques ranging from pharmaceutical production, bioethanol production along with medical to basic research.

In this lab, the student learns how to assemble an expression vector containing TetOff regulator, RAD52 and GFP. The aim is to control the expression level of RAD52 with Doxycyline and to monitor the expression level by observing the GFP signal.



Rasmus Frandsen, Bioengineering Associate Professor

Technical University of Denmark (DTU)

Learning Objectives

  • Understanding molecular cloning techniques: DNA extraction and preparation, ligation, transformation, plate streaking and antibiotic selection
  • Understanding inducible gene expression regulation (TetOff system)
  • Understanding the use of GFP as a reporter gene
  • Understanding DNA damage and DNA repair system


  • Tet-off gene
  • DNA extraction
  • Transformation
  • Colony screening
  • Cloning


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