Pipetting: Master the technique
About Pipetting: Master the technique Virtual Lab Simulation
In the Pipetting simulation, you will determine the amount of essential amino acids in genetically modified corn. Using a micropipette, you will prepare a serial dilution, and measure the protein concentration with a Bradford assay.
Get to know the micropipette
You will be introduced to the basics of pipetting as you dive right into the experiments. Step by step, you will learn how to adjust the volume, use the two ‘stops’ of the pipette, and find out which pipette to use for which volume range. You will also learn tips and tricks to make your pipetting more accurate.
Perform a serial dilution
Once you master the art of pipetting, you will dilute your protein solution 10.000 fold! While you progress through the lab, you will become more and more routined and independent. After this mission, you will understand the benefits of serial dilutions, and be able to perform one by yourself.
Measure the protein content in the sample
In this mission, you will dye your proteins blue, and use your new skills to prepare a microplate for a Bradford assay. In this colorimetric assay, you shine light of a specific wavelength through the plate, and measure the absorbance in each well. Finally, you will compare the absorbances of your sample to a sample of known protein concentration.
Will you be able to calculate the amount of essential amino acids in the corn cob you collected?
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Techniques In Lab
- Serial dilution
- Bradford assay
At the end of this simulation, you will be able to…
- Select the correct micropipette for its purpose
- Use the two stops of the pipette
- Explain pipetting techniques
- Perform a serial dilution
- Quantify the protein content in a sample with a Bradford assay
Screenshots of Pipetting: Master the techniqueVirtual Lab Simulation
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