Join Dr. One and two colleagues on their mission to cure cancer. They are using Western blot to compare the level of p53 protein in cancerous and healthy control cells. Can you help Dr. One with the tricky membrane transfer step?
University / College
Air bubbles are the enemy and if you are not careful you may cause a minor explosion! In this simulation you will learn how to set up and troubleshoot the transfer step of Western blot. Dr. One has already separated the proteins by size using gel electrophoresis. Before they can continue to use antibodies to detect the levels of p53 protein, the protein samples must be transferred from the gel to a membrane.
Building a stack
You will use electricity to transfer the proteins from the gel to the membrane. The gel and membrane must stay closely together during the whole procedure. To achieve this, you start by building the transfer stack. Be careful, as a mistake here could ruin your experiment! Luckily Dr. One is on hand to guide you.
Transferring the proteins
Once you have built the stack, you are ready to assemble the tank in which you will use electricity to move the proteins onto the membrane. As your colleagues Jenny and Mario will be able to tell you, even with the perfect stack, there is still the potential to cause a minor fire or other disaster at this point! Thankfully, the process is much quicker in the virtual lab as you do not need to wait for the transfer to complete.
Analyzing your results
Using antibodies to detect proteins is another lengthy process. Before handing your membrane over to Dr. One for this part of the Western blot experiment you want to make sure that you have been successful. You will use ponceau to stain all the proteins on the membrane so you can assess the quality of your transfer. Jenny and Mario have been working alongside you and need some help checking their membranes too. Can you find the best membranes for Dr. One to analyze?
At the end of this simulation, you will be able to:
Build a transfer stack with the correct orientation
Set up a transfer tank to transfer protein from an SDS-PAGE gel to a membrane.
Critique the quality of protein transfer using a removable protein-identifying stain.
At the end of this simulation, you will be able to:
Engage students in science through interactive learning scenarios. Simulate experiments, train lab techniques, and teach theory through visual experiences that enhance long-term learning outcomes.
300+ Web-based simulations that can be played on laptops, Chromebooks, and tablets/iPads without installing any software
Teacher dashboard to automate grading and track student progress
Embedded quizzes to help students master science content
Library of learning resources, lab reports, videos, theory pages, graphics and more
Learn how to develop or make products from living systems and organisms in our biotechnology lab simulations. Study how living organisms can be modified and used in fields such as pharmaceuticals and agriculture. You can also learn about what role genetically modified organisms play in the research of diseases such as cancer.
Learn how cells, organs and systems function in the human body. Study the science that underlies the understanding and treatment of human diseases, acquire skills in areas of biology and chemistry that are relevant to healthcare.
Learn about the microorganisms that are all around and inside us in our microbiology simulations. Study the living organisms that are too small to be seen with the naked eye, explore how to handle them in the laboratory, and discover how they play an important role in the causes and control of diseases.
Learn about chemical processes in living organisms in our biochemistry lab simulations. Study biological molecules and learn how they create the processes that occur within living cells. Discover how you get the energy you need for living, and how enzymes help every one of your body’s functions.
Learn about life and living organisms in our biology simulations for Majors. Study cells, genes and evolution and dive into the many layers of biology to discover what life is, and what it means for living beings’ existence on earth.
Learn about the basics of life and living organisms in our biology simulations for Non-majors. Study cells, genes and evolution and dive into the many layers of biology to discover what life is, and what it means for living beings’ existence on earth.
Learn about the structures and functions of the different types of cells as well as the biomolecules within them in our cellular and molecular biology lab simulations. Study the life cycles of the cell and how they interact with the environment. Discover how the knowledge of cells is fundamental to all biomedical sciences and is essential for research in fields such as cancer and other diseases.
Elevate your nursing program with UbiSim, a VR solution dedicated to clinical excellence.
Labster integrates with all major LMS (Learning Management Systems) so that educators can use their gradebooks to track students’ performance data and students can keep a record of their work. Labster is compatible with Canvas, Blackboard, Moodle, Google Classroom, Schoology, Sakai, and Brightspace / D2L. It’s also possible to use Labster without an LMS.