Cancer Pharmacology: Provide recommendations in a multi-million dollar project

Cancer Pharmacology
Time to complete course: 54 min.

Try our lab safety simulation

Discover one of 200+ learning simulations available today

About Cancer Pharmacology: Provide recommendations in a multi-million dollar project Virtual Lab Simulation

In this simulation, you will learn which tools are needed to screen thousands of chemical compounds in order to identify promising candidates for the treatment of different types of cancer. In particular, you will gain insight into the principles and limitations of widely used in vitro assays in the context of anti-cancer drug screenings. As a pharmacology expert, you will test the robustness of those commonly used techniques and help a screening facility to assess the risk for false negative results.

Prepare cells and drugs for chemosensitivity testing

Your main task in the Cancer Pharmacology simulation is to reproduce the screening facility’s chemosensitivity in vitro protocol to investigate the cause of false negative results. The protocol in question is a widely used cell survival assay using MTT reagent and performed with conventional cancer cell lines. The Project Leader suggests to perform the test with two well-known chemotherapy drugs: epirubicin and cyclophosphamide. In this part of the simulation, you will learn how to perform common steps of chemosensitivity testing and learn about basic pharmacology tools. Most importantly, you will count living cells of a cell culture solution and calculate how much of the culture you need for your experiment. You will also prepare stock solutions of both drugs with a given concentration to create a dilution series for the treatment of the cancer cells.

Perform the MTT assay

Once the cells have been prepared and incubated with the drugs, it is time to use MTT as a cell survival indicator. You will be able to follow on a molecular level how MTT specifically reacts with mitochondrial enzymes leading to a color colorimetric change that can be measured with a spectrophotometer. In this mission, you will work with a multi-channel pipette and 96 well plates to perform the assay as used in many pharmacology labs worldwide.

Determine EC50 and interpret your results

The result of the serial drug dilution in the MTT assay will provide the basis for creating a dose-response curve and for determining the half-maximal effective concentration (EC50) of both clinically used drugs. Which recommendations will you provide the Project Leader with about the usefulness of the commonly applied in vitro assays?

Get Started Now

Review the usefulness of a multi-million dollar operation to identify potential drugs for cancer chemotherapy, and provide your recommendations to the Project Leader.

Techniques in Lab

  • Cell counting
  • Drug treatment of cancer cells
  • MTT assay
  • EC50 determination

Learning Objectives

At the end of this simulation, you will be able to…

  • Understand the principles and limitations of chemosensitivity testing in the context of in vitro based anticancer drug screening
  • Conduct, analyze and interpret in vitro chemosensitivity tests using the MTT assay
  • Evaluate the advantages and disadvantages of chemosensitivity tests for in vitro based screening programs
  • Prepare cell cultures for chemosensitivity testing
  • Determine viable cell numbers using an automated cell counter
  • Conduct the MTT assay to assess cell survival following drug exposure
  • Generate dose response curves and determine half-maximal effective concentration (EC50) values
  • Interpret the results obtained in the chemosensitivity assay
  • Understand the mechanisms of action of cyclophosphamide and epirubicin

Simulation Features

Length – 54 minutes
Accessibility mode – Not Available
Languages – English

Examples of Related Standards


Pharmacology related 2nd & 3rd Year


No direct alignment


No direct alignment


No direct alignment

Screenshots of Cancer Pharmacology: Provide recommendations in a multi-million dollar project Virtual Lab Simulation


Prof. Roger Philips

Department of Pharmacy
University of Huddersfield

How it works

A million dollar lab in your browser

Perform experiments in virtual lab simulations to achieve core science learning outcomes. 

All our simulations run on laptop and desktop computers, and you can play our simulations without having to install any browser plugins.

See detailed minimum requirements here.

Hundreds of hours of science learning content

Our virtual laboratory simulations are aimed at university, college and high school level, within fields such as biology, biochemistry, genetics, biotechnology, chemistry, physics and more.

With access to our simulations, you will have hundreds of hours of engaging, high-quality learning content available to you.

Learn how you can use Labster in your science course

Take a 15 Minute “Lab Break” with a Virtual Lab Expert

Talk to one of our Virtual Lab Experts about how Labster can engage your students with our virtual labs for online, hybrid and face-to-face courses.

Lab break coffee with us

Please fill out the form below to talk with one of our Lab Experts. 

You must opt in to receive emails from Labster. You can unsubscribe at any time.
Thank you for your submission!

Sales Demo: Discover how Labster
improves student grades and learning outcomes!

Limited to 100 people

The simulation is already added to this package

Step 1: Choose your simulations

Find and select the simulations that you want to add to your course.

Step 2: Review simulation list

This is the list of simulations that will be added to your course. Click Download once you are ready.

Press the + button next to the simulations that you want to add to your list, or add all the simulations of this package by pressing “add all simulations”.


Related Simulations

No simulations added yet.
    Download the .zip file and upload it to your LMS.

    Download Common Cartridge File

    Choose your Learning Management System below:
    Generating download link. Please wait...
    Congratulations! You can now upload the .zip file into your LMS by the following these instructions:

    Simulation name

    Image container

    Learning Outcomes

    Learning outcomes container


    Techniques container

    Simulation Description

    Description container

    Are you sure you want to delete?

    Package deleted successfully

    IMPORTANT: Check the original email with instructions from your instructor to verify how you should gain access.

    Link Access

    Did you receive a link/URL to access Labster from your teacher? This is for either “Labster Direct” or “Quick Access”, and no login is required. See info below.

    School LMS

    Are you accessing Labster from your school’s LMS, such as Canvas, Blackboard, Moodle or Brightspace? Click below to read more.
    IMPORTANT: Please check your original email to confirm the correct access option.

    Labster Direct / Quick Access

    Did you receive a link/URL to access simulations through the Labster Direct or Quick Access pages? If so, no login is required. See info below.

    Your LMS

    Are you accessing Labster from your school’s LMS, such as Canvas, Blackboard, Moodle or Brightspace? Click below to read more.

    Faculty Resources

    To access the Faculty Resources page (instructors only) and review Labster simulations, please log in below.