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Confocal Microscopy

In this simulation, you will learn about the benefits of using a confocal microscope over other optical imaging techniques. Are you ready to identify the fungus and give your uncle advice on a potential treatment?

About This Simulation

In this simulation, you will learn about the benefits of using a confocal microscope over other optical imaging techniques. You will have a close look at the setup of this type of microscope and how to operate it. Finally, you will be trained in how to acquire pin-sharp micrographs and mind-blowing 3D renderings of your sample. Your overall task will be to use confocal microscopy to investigate a mystery plant disease found on a barley plant from your uncle’s crop fields. Are you ready to identify the fungus and give your uncle advice on a potential treatment?

Screenshots

Collaborators

Alice Carr, student collaborator

Exeter University

Claire Burgess, student collaborator

Exeter University

Georgina Mansell, student collaborator

Exeter University

Lucy Oram, student collaborator

Exeter University

Charlotte Robins, student collaborator

Exeter University

Ellie Sloman, student collaborator

Exeter University

Learning Objectives

  • Understanding the principles of confocal microscopy and how to perform it
  • Learning about the confocal microscope setup
  • Understanding the advantages of confocal microscopy over conventional optical microscopy
  • Learning how to acquire images and 3D renderings

 

Techniques

  • Laser scanning confocal microscopy
  • Staining of plant samples for fluorescence microscopy

 

Collaborator

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