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About This Simulation
Help a scientist detect and quantify proteins by using one of the most popular techniques in molecular biology.
Learning Objectives
- Explain the principle of different ELISA techniques
- Apply sandwich ELISA to quantify protein samples
- Analyze the standard curve of ELISA experiment
- Understand the function of reagents and equipment used in ELISA
- Describe the basic troubleshooting process of ELISA
About This Simulation
Lab Techniques
- ELISA
Related Standards
- No direct alignment
- No direct alignment
- No direct alignment
Learn More About This Simulation
Trying to capture a specific protein amongst thousands of types of proteins is like looking for a needle in a haystack. In the ELISA simulation, you will join scientists who are using a groundbreaking technique for detecting and quantifying substances, such as protein. The method is called Enzyme-linked immunosorbent assay (ELISA). You will help Dr. Lisa quantify Factor IX protein, which is used for hemophilia drugs.
Detecting Factor IX
In the simulation, Dr. Lisa is working on producing protein used for treating hemophilia patients. The protein is called Factor IX. She produces them in different cell lines. However, she needs your help to determine which cell lines produce Factor IX the most. To help Dr. Lisa, you will perform an ELISA technique.
Performing ELISA
Scientists have developed numerous kinds of ELISA. You will learn the principles of the most common ELISA method. ELISA is about antibody and antigen, which you should already be familiar with. Once you understand the principle of each type of ELISA, you will have to decide which ELISA technique you want to use.
Analyzing the results
Not all experiments run smoothly. In this simulation, you will compare good and bad results. By comparing the results, you will be able to perform basic troubleshooting for your results. Finally, you will interpolate your data to find out which cell lines are producing Factor IX the most.
Will you able to help Dr. Lisa in reducing the production cost of hemophilia drug?
Experience Labster for Yourself
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For Science Programs Providing a Learning Advantage
North Dakota State College of Science
“They did the simulation at home, then completed the in-person lab within 30 minutes, no questions asked, and passed the quiz with flying colors.”
Lecturer in Human Physiology
University of Westminster
"I saw some of the students who clearly didn’t necessarily like sitting there reading a book discover they could turn on Labster and keep up with the rest of the class because it spoke to them.
Modesto City Schools
"Having something that's engaging for the students gives teachers that opportunity to breathe and get excited again. Because they're seeing the kids light up, they're seeing the kids engage with content."
Wenatchee Valley College
"The question always is, ‘Can we demonstrate that the students are meeting course outcomes?’ Check! We can do that.”
San José State University
"We surveyed over 400 students. More than 90% thought Labster was easy to navigate, and that it was fun, but more importantly, most of them felt confident that they could execute the labs in person. And that confidence is a big deal."
FAQs
Find answers to frequently asked questions.
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Labster can be integrated within a school's LMS (Learning Management System), and students can access it like any other assignment in their LMS. If your Institution does not choose an LMS integration, students will log in to Labster's Course Manager once they have an account created. Your institution will decide the access method during the sales process.
Labster is only available for purchase by faculty and administration at academic institutions. To procure Labster, simply reach out to us on our website. Schedule a demo, book a meeting to discuss pricing, start a free trial, or simply fill out our contact form.
Labster simulations are created by real scientists and designed with unparalleled interactivity. Unlike point and click competitors, Labster simulations immerse students and encourage mastery through active learning.
Labster supports a wide range of courses at the high school and university level across fields in biology, chemistry and physics. Some simulations mimic lab procedures with high fidelity to train foundational skills, while others are meant to bring theory to life through interactive scenarios.