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Help a scientist detect and quantify proteins by using one of the most popular techniques in molecular biology.

About This Simulation

Trying to capture a specific protein amongst thousands of types of proteins is like looking for a needle in a haystack. In the ELISA simulation, you will join scientists who are using a groundbreaking technique for detecting and quantifying substances, such as protein. The method is called Enzyme-linked immunosorbent assay (ELISA). You will help Dr. Lisa quantify Factor IX protein, which is used for hemophilia drugs.

Detecting Factor IX
In the simulation, Dr. Lisa is working on producing protein used for treating hemophilia patients. The protein is called Factor IX. She produces them in different cell lines. However, she needs your help to determine which cell lines produce Factor IX the most. To help Dr. Lisa, you will perform an ELISA technique.

Performing ELISA
Scientists have developed numerous kinds of ELISA. You will learn the principles of the most common ELISA method. ELISA is about antibody and antigen, which you should already be familiar with. Once you understand the principle of each type of ELISA, you will have to decide which ELISA technique you want to use.

Analyzing the results
Not all experiments run smoothly. In this simulation, you will compare good and bad results. By comparing the results, you will be able to perform basic troubleshooting for your results. Finally, you will interpolate your data to find out which cell lines are producing Factor IX the most. Will you able to help Dr. Lisa in reducing the production cost of hemophilia drug?



Learning Objectives

At the end of this simulation, you will be able to….

  • Explain the principle of different ELISA techniques
  • Apply sandwich ELISA to quantify protein samples
  • Analyze the standard curve of ELISA experiment
  • Understand the function of reagents and equipment used in ELISA
  • Describe the basic troubleshooting process of ELISA

Techniques In This Simulation

  • Sandwich ELISA

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