About Synthetic Biology Virtual Lab Simulation
Use the specific microRNA profile of cancer cells to design an apoptotic biological circuit that is only activated in cancer cells. You are the only hope for a patient with a rare form of cancer.
Learn how to use the Gateway cloning technique
In the Synthetic Biology simulation, you will learn how to use the Gateway cloning technique to combine different genetic modules in an expression vector. You will learn how the Gateway cloning technique can be used to efficiently combine these modules. Use electroporation to transform the bacteria with your vector of interest and select successful transformants. You will learn all about the basics of sterile lab work and bacterial selection.
Extract and isolate plasmids
Your next task in the lab will be to grow the transformed bacteria and extract the plasmids using a ‘miniprep’ kit. You will learn how different buffers enable you to isolate plasmid, and not genomic, DNA from the cells. You will check if the plasmid, indeed, contains your circuit, and if it is mutation-free by digesting it with restriction enzymes and by using gel electrophoresis. In the end you will test your circuit in living cells to see if the cancer cells are the only cells that die.
Will you be able to find a cure for this rare form of cancer?
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Techniques In Lab
- Gateway cloning technique
- Antibiotics selection
- Sterile technique
- Plasmid isolation with purification columns
- Restriction digest
- Gel electrophoresis
At the end of this simulation, you will be able to…
- Engineer natural systems to perform specific functions
- Describe the fundamentals of the Gateway cloning technique and design your own biological circuit
- Explain and perform bacterial transformation, antibiotic selection and plasmid purification
- Explain and perform a restriction digest of your cloning product
Screenshots of Synthetic Biology Virtual Lab Simulation
Prof. Ron WeissProfessor of Biological Engineering Synthetic Biology Center at MIT
Shiva Kalinga, PhDManaging Director Synthetic Biology Center at MIT
How it works
A million dollar lab in your browser
Perform experiments in virtual lab simulations to achieve core science learning outcomes.
All our simulations run on laptop and desktop computers, and you can play our simulations without having to install any browser plugins.
Hundreds of hours of science learning content
Our virtual laboratory simulations are aimed at university, college and high school level, within fields such as biology, biochemistry, genetics, biotechnology, chemistry, physics and more.
With access to our simulations, you will have hundreds of hours of engaging, high-quality learning content available to you.