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About This Simulation
In the Fluorescence-automated Cell Sorting (FACS) Simulation, you will learn the basics of flow cytometry and find out how to use a flow cytometer with fluorescence detection.
Learning Objectives
- Understand the basics of flow cytometry technique
- Understand the importance of each system that the technique relies on (fluidics, optics, and electronics)
- Use a cell sorter (start up, sorting, and shut down of the equipment)
- Understand how the equipment performs the measurements
- Interpret the results and understand the applications of the technique
About This Simulation
Lab Techniques
- Flow cytometry
Related Standards
Learn More About This Simulation
Fluorescence-activated cell sorting (FACS) is a specialized type of flow cytometry. It provides a method for sorting a heterogeneous mixture of cells one at a time, based on the specific light scattering and fluorescent characteristics of each cell. It provides fast, objective, and quantitative recording of fluorescent signals from individual cells, as well as physical separation of cells of particular interest.
Learn about FACS
Using the light scattering, FACS can detect cell size and complexity, while the fluorescence characteristics allow researchers to analyze different biological functions or study specific cell components. As a flow cytometry technique, FACS is also based on three main systems: fluidics, optics, and electronics. Their combined work allows the process of cell sorting.
Use your FACS knowledge to sort GFP
In the FACS simulation, you will learn the basics of flow cytometry and find out how to use a flow cytometer with fluorescence detection. Your goal is to sort a pool of Escherichia coli expressing Green Fluorescent Protein (GFP) at different levels after it has been previously engineered. You will set up the equipment, learn how the cytometer sorts the cells, interpret your results, and clean and shut down the equipment.
Will you be able to perform all the steps correctly and sort the cells depending on their GFP expression level?
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FAQs
Find answers to frequently asked questions.
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A Labster virtual lab is an interactive, multimedia assignment that students access right from their computers. Many Labster virtual labs prepare students for success in college by introducing foundational knowledge using multimedia visualizations that make it easier to understand complex concepts. Other Labster virtual labs prepare learners for careers in STEM labs by giving them realistic practice on lab techniques and procedures.
Labster’s virtual lab simulations are created by scientists and designed to maximize engagement and interactivity. Unlike watching a video or reading a textbook, Labster virtual labs are interactive. To make progress, students must think critically and solve a real-world problem. We believe that learning by doing makes STEM stick.
Yes, Labster is compatible with all major LMS (Learning Management Systems) including Blackboard, Canvas, D2L, Moodle, and many others. Students can access Labster like any other assignment. If your institution does not choose an LMS integration, students will log into Labster’s Course Manager once they have an account created. Your institution will decide which is the best access method.
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Labster supports a wide range of STEM courses at the high school, college, and university level across fields in biology, chemistry, physics, and health sciences. You can identify topics for your courses by searching our Content Catalog.