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About This Simulation
Perform the chromatin immunoprecipitation with exonuclease treatment (ChIP-exo) protocol, and prepare the target DNA to learn how E. coli survive in acid stress conditions.
Learning Objectives
- Understand the use of exonuclease treatment in ChIP-exo technique.
- Understand the key steps for performing the ChIP-exo technique as well as the importance of each reagent:
- Adding formaldehyde to the culture to fix the bonds between proteins and DNA.
- Lysing the cells using lysozyme and sonication.
- Addition of specific antibodies.
- Use of magnetic beads.
- On-bead enzymatic modifications.
- Off-bead enzymatic modifications.
- Understand the molecular mechanism of ChIP-exo.
About This Simulation
Lab Techniques
- DNA editing
- PCR
- Immunodetection
- Sonication
- Cell lysis
- Immunoprecipitation
Related Standards
- No direct alignment
- No direct alignment
- No direct alignment
Learn More About This Simulation
In the ChIP-exo simulation, you will perform the chromatin immunoprecipitation with exonuclease treatment (ChIP-exo) protocol in order to get a DNA sequence to elucidate the acid stress mechanism of Escherichia coli. You will learn how to fix the protein-DNA interactions and lyse the cells using a sonicator, add magnetic beads to the sample, and perform different on-bead and off-bead enzymatic modifications. From this, you will learn how the exonucleases work.
Will you be able to prepare the target DNA to understand how E. coli survive in acid stress conditions?
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FAQs
Find answers to frequently asked questions.
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A Labster virtual lab is an interactive, multimedia assignment that students access right from their computers. Many Labster virtual labs prepare students for success in college by introducing foundational knowledge using multimedia visualizations that make it easier to understand complex concepts. Other Labster virtual labs prepare learners for careers in STEM labs by giving them realistic practice on lab techniques and procedures.
Labster’s virtual lab simulations are created by scientists and designed to maximize engagement and interactivity. Unlike watching a video or reading a textbook, Labster virtual labs are interactive. To make progress, students must think critically and solve a real-world problem. We believe that learning by doing makes STEM stick.
Yes, Labster is compatible with all major LMS (Learning Management Systems) including Blackboard, Canvas, D2L, Moodle, and many others. Students can access Labster like any other assignment. If your institution does not choose an LMS integration, students will log into Labster’s Course Manager once they have an account created. Your institution will decide which is the best access method.
Labster is available for purchase by instructors, faculty, and administrators at education institutions. Purchasing our starter package, Labster Explorer, can be done using a credit card if you are located in the USA, Canada, or Mexico. If you are outside of North America or are choosing a higher plan, please speak with a Labster sales representative. Compare plans.
Labster supports a wide range of STEM courses at the high school, college, and university level across fields in biology, chemistry, physics, and health sciences. You can identify topics for your courses by searching our Content Catalog.